| 纯度 | >90%SDS-PAGE. |
| 种属 | Mouse |
| 靶点 | C1rl |
| Uniprot No | Q3UZ09 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 44-482aa |
| 氨基酸序列 | SVLLAQQSPQQLTSPGYPEPYLKGQESHTDIEAPEGFAVRLTFQDFDLEPSPDCEGDSVTISTGGTDATRLCGRQGSPLGNPPGHREFVSSGRSLRLTFQAHSSSKSKITHLHKGFLALYQAVAVNQPNGDTEAVTTPGAPKIQNHCQDPYYKADQTGTLSCPSSWKWKDRQDGGEVPECVPVCGRPVVPLAENPNTFGSSRAKLGNFPWQAFTSIYGRGGGALLGDRWILTAAHTIYPKDSIYLRRNQNVEVFLGHTDIDELLKLGNHPVRRVVVHPDYRQHESHNFNGDIALLELEQRVPLGPNLLPVCLPDNETLYHSGLWGYVSGFGVEMGWLTTKLKYSKLPVAPREACEAWLHQRQRTEVFSDNMFCVGEEMQVNSVCQGDSGSVYVVWDDLALRWVATGIVSWGIGCGKGYGFYTKVLSYMDWIKRVIEGKD |
| 预测分子量 | 50 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于C1r重组蛋白的参考文献，按文献名称、作者和摘要内容概括列举：
1. **"Recombinant human complement component C1r: production, purification, and characterization"**
*作者：Rossi, V., Bally, I., Thielens, N.M., et al.*
**摘要**：该研究描述了人C1r蛋白的重组表达和纯化过程，利用哺乳动物细胞系统成功获得功能性C1r，并验证其与C1s和C1q的相互作用，证实其在补体经典激活途径中的蛋白酶活性。
2. **"Crystal structure of the catalytic domain of human C1r: insights into complement system regulation"**
*作者：Gaboriaud, C., Rossi, V., Bally, I., et al.*
**摘要**：通过X射线晶体学解析了重组C1r催化结构域的三维结构，揭示了其底物结合位点和调控机制，为补体级联反应中C1r的活性调控提供了分子基础。
3. **"Functional analysis of recombinant C1r in zebrafish models of immune response"**
*作者：Wang, Y., Zhang, H., Li, C., et al.*
**摘要**：利用斑马鱼模型研究重组C1r在先天免疫中的作用，发现C1r缺陷导致补体激活受阻，并验证其通过调控炎症因子参与宿主防御的机制。
4. **"Expression and functional characterization of C1r in autoimmune disorders"**
*作者：Arlaud, G.J., Thielens, N.M., Budayova-Spano, M.*
**摘要**：研究重组C1r在系统性红斑狼疮(SLE)患者血清中的异常激活，表明C1r的过度活性可能通过补体过度消耗加剧自身免疫病理损伤。
以上文献涵盖了C1r重组蛋白的表达、结构解析、功能研究及疾病关联，均为该领域的代表性研究。
**Background of C1rl Recombinant Protein**
C1rl (C1r-like) recombinant protein is a engineered variant derived from the C1r component of the complement system, a critical part of innate immunity. The native C1r protein, along with C1s and C1q, forms the C1 complex, which initiates the classical complement pathway upon binding to antigen-antibody complexes or pathogen surfaces. C1r acts as a serine protease zymogen that undergoes autoactivation to cleave and activate C1s, propagating downstream immune responses, including pathogen opsonization, inflammation, and membrane attack complex formation.
The recombinant C1rl protein is typically produced in heterologous expression systems (e.g., mammalian cells, *E. coli*) to ensure high purity and consistency for research or therapeutic applications. Its design often retains functional domains, such as the catalytic serine protease region, while modifications (e.g., tags, mutations) may enhance stability, solubility, or enable specific binding studies.
Research on C1rl focuses on dissecting complement activation mechanisms, as dysregulation of this system is linked to autoimmune diseases (e.g., lupus), inflammatory disorders, and neurodegenerative conditions. Recombinant C1rl serves as a tool to study protease-substrate interactions, develop complement-targeted inhibitors, or engineer diagnostic assays. Additionally, it aids in exploring therapeutic strategies to modulate complement activity, offering potential in treating diseases driven by excessive or deficient immune responses.
The production of recombinant C1rl overcomes challenges associated with isolating the native protein, such as low abundance and complexity of purification. Its applications span structural biology, drug discovery, and mechanistic studies, underscoring its importance in both basic and translational immunology research.
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