| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | iceE |
| Uniprot No | P16239 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1129-1258aa |
| 氨基酸序列 | MAGERGKLIAGADSTQTAGDRSKLLAGNNSYLTAGDRSKLTAGNDCILMAGDRSKLTAGINSILTAGCRSKLIGSNGSTLTAGENSVLIFRCWDGKRYTNVVAKTGKGGIEADMPYQMDEDNNIVNKPEE |
| 预测分子量 | 15.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是模拟生成的关于IceE重组蛋白的参考文献示例(仅供学术参考，非真实文献)：
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1. **《Expression and characterization of recombinant IceE protein from Pseudomonas syringae》**
*作者：Lee, J., et al.*
摘要：研究在大肠杆菌中重组表达冰核蛋白IceE，优化诱导条件后获得高纯度蛋白，并通过差示扫描量热法证实其冰核活性，为低温生物技术应用提供基础。
2. **《Enhancing freeze tolerance in lactic acid bacteria using IceE fusion proteins》**
*作者：Zhang, H., et al.*
摘要：将IceE与乳酸菌表面蛋白融合表达，显著提升菌体在冷冻过程中的存活率，证明其在食品工业冷冻保存中的潜在应用价值。
3. **《Structural insights into the ice-binding mechanism of IceE protein via cryo-EM》**
*作者：Martinez, R., et al.*
摘要：通过冷冻电镜解析IceE蛋白的三维结构，发现其重复结构域与冰晶表面氢键的特异性结合模式，揭示了冰核形成的分子机制。
4. **《Directed evolution of IceE for improved ice nucleation efficiency》**
*作者：Kumar, S., et al.*
摘要：利用定向进化技术对IceE基因进行突变筛选，获得突变体在-5℃下的冰核活性提高3倍，为人工雪制备和气候研究提供改良工具。
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提示：实际文献需通过PubMed/Web of Science等平台以关键词“IceE recombinant protein”或“Ice nucleation protein engineering”检索。
IceE recombinant protein is a engineered biomolecule derived from ice-binding proteins (IBPs) originally identified in extremophilic organisms, such as Antarctic bacteria or cold-adapted fungi. These natural IBPs enable survival in subzero environments by inhibiting ice crystal growth and recrystallization, a mechanism critical for protecting cellular structures from freeze damage. The IceE variant was developed to enhance stability, solubility, and functional versatility for biotechnological applications, addressing limitations of native IBPs like low yield and thermal instability.
Produced through recombinant DNA technology, the IceE gene is cloned into expression vectors (e.g., E. coli or yeast systems) and purified using affinity chromatography. Its modular design often includes fusion tags for easier purification or functional coupling. Structurally, IceE retains the repetitive amino acid motifs of natural IBPs that interact with ice nuclei via hydrogen bonding and surface complementarity, but engineered mutations may optimize ice-binding affinity or adjust thermal hysteresis activity.
Research on IceE focuses on cryopreservation (e.g., organs, vaccines, and food), antifreeze coatings for infrastructure, and controlled ice templating in material science. In biomedicine, it shows promise for improving cell viability during frozen storage. Industrial applications include ice cream texture modification and frost-resistant coatings. Current challenges involve balancing its ice-inhibition efficiency with production costs and resolving potential immunogenicity in therapeutic contexts. Ongoing studies explore structure-function relationships through computational modeling and directed evolution to expand its practical utility in cryobiology and beyond.
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