| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FTCD |
| Uniprot No | O95954 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-541aa |
| 氨基酸序列 | MSQLVECVPNFSEGKNQEVIDAISGAITQTPGCVLLDVDAGPSTNRTVYTFVGPPECVVEGALNAARVASRLIDMSRHQGEHPRMGALDVCPFIPVRGVSVDECVLCAQAFGQRLAEELDVPVYLYGEAARMDSRRTLPAIRAGEYEALPKKLQQADWAPDFGPSSFVPSWGATATGARKFLIAFNINLLGTKEQAHRIALNLREQGRGKDQPGRLKKVQGIGWYLDEKNLAQVSTNLLDFEVTALHTVYEETCREAQELSLPVVGSQLVGLVPLKALLDAAAFYCEKENLFILEEEQRIRLVVSRLGLDSLCPFSPKERIIEYLVPERGPERGLGSKSLRAFVGEVGARSAAPGGGSVAAAAAAMGAALGSMVGLMTYGRRQFQSLDTTMRRLIPPFREASAKLTTLVDADAEAFTAYLEAMRLPKNTPEEKDRRTAALQEGLRRAVSVPLTLAETVASLWPALQELARCGNLACRSDLQVAAKALEMGVFGAYFNVLINLRDITDEAFKDQIHHRVSSLLQEAKTQAALVLDCLETRQE |
| 预测分子量 | 64.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FTCD重组蛋白的3篇示例参考文献(内容为模拟示例，供参考)：
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1. **文献名称**：*Expression and purification of recombinant human FTCD in Escherichia coli*
**作者**：Li, X., Chen, J., & Wang, Y.
**摘要**：本研究成功克隆并表达了人源FTCD重组蛋白于大肠杆菌系统，通过亲和层析纯化获得高纯度蛋白。实验验证了重组FTCD的酶活性，为后续功能研究提供了工具。
2. **文献名称**：*Structural insights into FTCD-mediated folate metabolism by cryo-EM*
**作者**：Smith, R.L., Brown, T.P., & Davies, K.
**摘要**：利用冷冻电镜技术解析了FTCD重组蛋白的三维结构，揭示了其催化结构域与底物结合的关键位点，阐明了其在叶酸代谢循环中的分子机制。
3. **文献名称**：*FTCD deficiency exacerbates metabolic disorders in a mouse model*
**作者**：Kim, S., Park, H., & Tanaka, M.
**摘要**：通过构建FTCD敲除小鼠模型，发现FTCD重组蛋白缺失导致组氨酸代谢异常，并加剧高脂饮食诱导的肝脏脂肪变性，提示FTCD在代谢疾病中的潜在调控作用。
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**提示**：实际文献需通过PubMed、Google Scholar等平台检索，推荐关键词：
`FTCD recombinant protein expression`、`formimidoyltransferase cyclodeaminase structure/function`、`FTCD metabolic role`。
**Background of FTCD Recombinant Protein**
FTCD (Formiminotransferase Cyclodeaminase) is a bifunctional enzyme encoded by the *FTCD* gene, primarily involved in histidine catabolism and folate-mediated one-carbon metabolism. It catalyzes two sequential reactions: the transfer of a formimino group from formiminoglutamate (FIGLU) to tetrahydrofolate (THF), followed by cyclodeamination to produce glutamate and 5.10-methenyl-THF. These reactions link amino acid metabolism with folate cycles, essential for nucleotide synthesis, methylation processes, and cellular redox regulation.
The interest in recombinant FTCD stems from its role in metabolic disorders and disease mechanisms. For instance, FTCD dysfunction has been implicated in hyperhistidinemia and folate deficiency-related conditions. Recombinant FTCD protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cell cultures), enables detailed biochemical and structural studies. Its purification allows researchers to analyze enzyme kinetics, substrate specificity, and interactions with metabolic partners, aiding in the understanding of folate-dependent pathways.
Additionally, FTCD has been explored as a potential autoantigen in autoimmune hepatitis type 2 (AIH-2), where anti-FTCD antibodies are detected in patients. Recombinant FTCD serves as a critical tool for developing diagnostic assays and studying autoimmune pathogenesis. Structural insights from recombinant FTCD also support drug discovery efforts targeting folate metabolism in cancers or microbial infections.
Overall, FTCD recombinant protein bridges fundamental biochemistry with translational research, offering avenues to dissect metabolic regulation, diagnose diseases, and design therapeutic interventions. Its study underscores the interplay between amino acid and folate metabolism in health and disease.
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