| WB | 1/500 - 1/2000 | Human,Monkey |
| IF | 咨询技术 | Human,Monkey |
| IHC | 1/200 - 1/1000 | Human,Monkey |
| ICC | 1/200 - 1/1000 | Human,Monkey |
| FCM | 1/200 - 1/400 | Human,Monkey |
| Elisa | 1/10000 | Human,Monkey |
| Aliases | HSSB; RF-A; RP-A; REPA1; RPA70; MST075 |
| Entrez GeneID | 6117 |
| clone | 4C4 |
| WB Predicted band size | 68.1kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Monkey |
| Immunogen | Purified recombinant fragment of human RPA1 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于RPA1抗体的3篇参考文献的简要整理：
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1. **文献名称**：*RPA1 binding to NRF2 switches ARE-dependent transcriptional activation to ARE-NRE–dependent repression*
**作者**：Hiroshi Takagi et al.
**摘要**：本研究揭示了RPA1通过结合抗氧化反应元件(ARE)抑制NRF2的转录活性，在氧化应激调控中起关键作用。实验中利用RPA1抗体进行ChIP-seq和免疫共沉淀，证实了RPA1与DNA损伤响应通路的关联。
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2. **文献名称**：*Replication Protein A (RPA1) in Homologous Recombination*
**作者**：Youngho Kwon et al.
**摘要**：文章通过免疫荧光和Western blot(使用RPA1抗体)证明RPA1在DNA同源重组修复中的功能，发现其缺失会导致修复效率下降，并影响BRCA2介导的修复机制。
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3. **文献名称**：*RPA1 phosphorylation in cancer cell survival and therapy resistance*
**作者**：Maria A. Vassin et al.
**摘要**：研究利用RPA1抗体分析磷酸化修饰对肿瘤细胞存活的影响，发现化疗药物诱导的RPA1磷酸化通过ATR通路增强癌细胞对DNA损伤的耐受性，为靶向治疗提供新方向。
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如有具体研究场景需求，可进一步筛选更匹配的文献!
The Replication Protein A1 (RPA1) antibody is a critical tool for studying the RPA complex, a single-stranded DNA (ssDNA)-binding protein essential for DNA replication, repair, and recombination. RPA1. the largest subunit (70 kDa) of the heterotrimeric RPA complex (comprising RPA1. RPA2. and RPA3), stabilizes ssDNA during processes like replication fork progression, nucleotide excision repair, and homologous recombination. It also coordinates checkpoint signaling and recruitment of DNA-processing enzymes. Dysregulation of RPA1 is linked to genomic instability, cancer progression, and resistance to therapies targeting DNA damage.
RPA1 antibodies are widely used in molecular biology to detect RPA1 expression via techniques such as Western blotting, immunofluorescence, and immunoprecipitation. They help assess RPA1’s role in cell cycle regulation, DNA damage response (e.g., after UV or chemotherapy), and interactions with partners like p53 or BRCA1/2. Researchers also employ these antibodies to investigate RPA1 overexpression in cancers (e.g., breast, lung) as a potential biomarker for prognosis or therapeutic targeting. Specificity is validated using knockout cell lines or competitive peptides.
Overall, RPA1 antibodies are indispensable for elucidating DNA metabolism mechanisms and developing strategies to exploit RPA1 dysfunction in disease contexts.
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