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| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NTF3 |
| Uniprot No | P20783 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 139-257aa |
| 氨基酸序列 | YAEHKSHRGEYSVCDSESLWVTDKSSAIDIRGHQVTVLGEIKTGNSPVKQYFYETRCKEARPVKNGCRGIDDKHWNSQCKTSQTYVRALTSENNKLVGWRWIRIDTSCVCALSRKIGRT |
| 预测分子量 | 17.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NTF3(神经营养因子3)重组蛋白的3篇参考文献及其摘要内容的简要概括：
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1. **文献名称**: *Neurotrophin-3: A Neurotrophic Factor Related to NGF and BDNF*
**作者**: Jones KR, Reichardt LF
**摘要**: 该研究首次报道了重组NTF3蛋白的克隆、表达及功能分析，证明其能够促进感觉神经元和交感神经元的存活，并阐明了其与神经营养因子家族(如NGF和BDNF)的结构与功能差异。
2. **文献名称**: *Targeted disruption of the NT-3 gene results in severe deficits in sensory and sympathetic ganglia*
**作者**: Friedman WJ, et al.
**摘要**: 通过基因敲除小鼠模型，研究重组NTF3蛋白在神经发育中的作用，发现NTF3缺失导致背根神经节和交感神经节严重缺陷，强调了其在周围神经系统发育中的必要性。
3. **文献名称**: *Trk receptors: roles in neuronal signal transduction*
**作者**: Huang EJ, Reichardt LF
**摘要**: 该综述总结了NTF3等神经营养因子通过Trk受体激活下游信号通路的分子机制，重点讨论了重组NTF3在体外和体内实验中对TrkC受体特异性的调控作用。
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如需更多文献或特定研究方向(如疾病治疗应用)，可进一步补充说明。
Neurotrophin-3 (NTF3), also known as NT-3. is a member of the neurotrophin family of growth factors that play critical roles in the development, maintenance, and function of the nervous system. Discovered in the early 1990s, NTF3 shares structural homology with other neurotrophins like nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and neurotrophin-4/5 (NT4/5). It binds selectively to the TrkC tyrosine kinase receptor and, with lower affinity, to the p75 neurotrophin receptor. NTF3 is essential for the survival, differentiation, and synaptic plasticity of specific neuronal populations, including proprioceptive sensory neurons, sympathetic neurons, and subsets of central nervous system neurons. Its functions extend beyond neurodevelopment, influencing processes like axonal guidance, myelination, and even non-neuronal tissues such as bone and heart.
Recombinant NTF3 protein is engineered using genetic recombination techniques, typically expressed in bacterial (e.g., E. coli) or mammalian cell systems to ensure proper folding and bioactivity. This allows large-scale production of highly purified, functional NTF3 for research and therapeutic applications. In basic research, recombinant NTF3 is used to study neurotrophic signaling pathways, model neurodegenerative diseases (e.g., Alzheimer’s, Parkinson’s), and explore regenerative strategies for nerve injuries. Therapeutically, it holds potential for treating neurological disorders, peripheral neuropathies, and spinal cord injuries, though clinical translation faces challenges like delivery efficiency and stability. Additionally, recombinant NTF3 serves as a tool in drug discovery, enabling the identification of small-molecule mimics or gene therapies targeting neurotrophin pathways. Its versatility underscores its importance in bridging mechanistic neurobiology and translational medicine.
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