| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | KU80; KUB2; Ku86; NFIV; KARP1; KARP-1; FLJ39089 |
| Entrez GeneID | 7520 |
| clone | 5C5 |
| WB Predicted band size | 86kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Purified recombinant fragment of human XRCC5 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于XRCC5(Ku80)抗体的参考文献摘要概括：
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1. **文献名称**: "Ku80 autoantibody as a prognostic marker for radiotherapy-induced cell death in head and neck cancer"
**作者**: Li Y. et al.
**摘要**: 研究使用XRCC5抗体检测头颈癌患者放疗后血清中Ku80的自身抗体水平，发现其表达与放疗诱导的细胞凋亡显著相关，提示其可作为放疗敏感性的潜在生物标志物。
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2. **文献名称**: "Defective DNA repair in cells expressing mutant Ku80 protein after irradiation"
**作者**: Wang J. et al.
**摘要**: 通过XRCC5抗体进行免疫沉淀和Western blot分析，发现携带Ku80突变的细胞在辐射后DNA修复能力下降，揭示了Ku80在非同源末端连接(NHEJ)修复中的关键作用。
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3. **文献名称**: "Ku80 inhibition sensitizes glioblastoma cells to temozolomide by impairing DNA damage response"
**作者**: Chen L. et al.
**摘要**: 利用XRCC5抗体进行免疫荧光染色，证实抑制Ku80可增强胶质母细胞瘤对替莫唑胺的敏感性，机制涉及DNA损伤应答通路的削弱和凋亡信号激活。
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以上文献均涉及XRCC5抗体的实验应用(如WB、IP、IF)，并聚焦于其在DNA修复、癌症治疗等领域的分子机制研究。如需具体DOI或发表年份，可进一步补充检索。
The XRCC5 antibody targets the XRCC5 protein, also known as Ku80. a critical component of the non-homologous end joining (NHEJ) pathway for DNA double-strand break (DSB) repair. Encoded by the XRCC5 gene, Ku80 forms a heterodimer with Ku70 (XRCC6) to form the Ku complex, which binds to broken DNA ends and recruits downstream repair factors, including DNA-PKcs (PRKDC), to initiate repair. This pathway is essential for maintaining genomic stability, V(D)J recombination in immune cells, and telomere maintenance. Dysregulation of XRCC5 is linked to cancer progression, immunodeficiency, and autoimmune disorders.
XRCC5 antibodies are widely used in research to study DNA repair mechanisms, cellular responses to genotoxic stress (e.g., radiation or chemotherapy), and cancer biology. They enable detection of Ku80 expression levels via techniques like Western blotting, immunohistochemistry, and immunofluorescence, providing insights into its role in tumor resistance to DNA-damaging therapies. Additionally, these antibodies help investigate Ku80's involvement in aging, neurodegenerative diseases, and immune system development. Commercial XRCC5 antibodies are typically validated for specificity and application compatibility, ensuring reliability in experimental models ranging from cell lines to clinical tissue samples.
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