| WB | 1/500 - 1/2000 | Human,Mouse,Rat,,Monkey |
| IF | 咨询技术 | Human,Mouse,Rat,,Monkey |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat,,Monkey |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat,,Monkey |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat,,Monkey |
| Elisa | 1/10000 | Human,Mouse,Rat,,Monkey |
| Aliases | AGM4; BASH; LY57; SLP65; BLNK-S; SLP-65; MGC111051 |
| Entrez GeneID | 29760 |
| clone | 5G9 |
| WB Predicted band size | 68kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat,,Monkey |
| Immunogen | Purified recombinant fragment of human BLNK expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于BLNK抗体的3-4篇参考文献示例(文献为模拟，供参考)：
---
1. **文献名称**: *BLNK: A Central Scaffold Protein in B Cell Receptor Signaling*
**作者**: Fu, C., et al.
**摘要**: 本研究通过BLNK特异性抗体，揭示了BLNK在B细胞受体(BCR)信号中的支架作用。实验表明，BLNK通过招募PLCγ2和Vav等信号分子，调控钙离子流动和细胞骨架重组，是B细胞活化的关键中介。
2. **文献名称**: *Impaired B Cell Development in BLNK-Deficient Mice*
**作者**: Ishiai, M., et al.
**摘要**: 利用BLNK抗体检测基因敲除小鼠模型，发现BLNK缺失导致前B细胞向成熟B细胞分化停滞，证实BLNK在B细胞发育中不可或缺，并影响免疫球蛋白重链的编辑。
3. **文献名称**: *BLNK Mediates Syk-Dependent Signaling Pathways in B Cells*
**作者**: Kurosaki, T., et al.
**摘要**: 通过共免疫沉淀实验(使用BLNK抗体)，阐明BLNK与Syk激酶的结合促进下游MAPK和NF-κB通路的激活，为B细胞增殖和存活提供分子机制依据。
4. **文献名称**: *BLNK Expression Correlates with Autoimmunity Severity*
**作者**: Wang, L., et al.
**摘要**: 在系统性红斑狼疮(SLE)患者中，采用BLNK抗体进行免疫组化分析，发现BLNK在过度活跃的B细胞中高表达，提示其可作为疾病生物标志物或治疗靶点。
---
**注**：以上文献为示例性内容，实际引用时需核实真实文献来源及准确性。建议通过PubMed或Google Scholar以关键词“BLNK antibody”或“BLNK signaling”检索最新研究。
**Background of BLNK Antibody**
BLNK (B-cell linker protein), also known as SLP-65 or BASH, is a critical adaptor protein in B-cell receptor (BCR) signaling. It is encoded by the *BLNK* gene and primarily expressed in B lymphocytes. BLNK plays a pivotal role in bridging activated BCR-associated kinases (e.g., Syk) to downstream signaling pathways, including PLCγ activation, calcium mobilization, and MAPK/NF-κB regulation. This interaction is essential for B-cell development, differentiation, and immune response coordination.
BLNK deficiency disrupts BCR signal transduction, leading to impaired B-cell maturation and antibody production. Mutations in *BLNK* are linked to agammaglobulinemia, a primary immunodeficiency characterized by low antibody levels and recurrent infections. Additionally, aberrant BLNK expression has been observed in B-cell malignancies, suggesting its role in oncogenic signaling.
BLNK-specific antibodies are vital tools for studying B-cell biology and pathology. They enable detection of BLNK expression, phosphorylation status, and interactions in immune assays (e.g., Western blot, immunoprecipitation). Research applications include investigating immune disorders, leukemia mechanisms, and therapeutic targets for modulating B-cell activity. These antibodies also aid in diagnosing genetic defects affecting BCR signaling pathways.
In summary, BLNK antibodies provide insights into B-cell function, disease mechanisms, and potential immunotherapies, highlighting their importance in both basic and clinical immunology.
×
