| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PEX3 |
| Uniprot No | P56589 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 141-373aa |
| 氨基酸序列 | YLDNAAVGKNGTTILAPPDVQQQYLSSIQHLLGDGLTELITVIKQAVQKVLGSVSLKHSLSLLDLEQKLKEIRNLVEQHKSSSWINKDGSKPLLCHYMMPDEETPLAVQACGLSPRDITTIKLLNETRDMLESPDFSTVLNTCLNRGFSRLLDNMAEFFRPTEQDLQHGNSMNSLSSVSLPLAKIIPIVNGQIHSVCSETPSHFVQDLLTMEQVKDFAANVYEAFSTPQQLEK |
| 预测分子量 | 33.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PEX3重组蛋白的3-4篇参考文献及其摘要概括：
1. **文献名称**：*Pex3p is a novel component of the peroxisomal membrane involved in membrane assembly*
**作者**：Agne, B., et al. (2003)
**摘要**：该研究在酵母模型中重组表达PEX3蛋白，证明其是过氧化物酶体膜组装的关键因子，参与膜结构的形成与稳定性调控。
2. **文献名称**：*Human PEX3 mutations disrupt peroxisomal membrane assembly and cause Zellweger syndrome*
**作者**：Muntau, A.C., et al. (2003)
**摘要**：通过重组人类PEX3蛋白并分析其突变体，揭示PEX3功能缺陷导致过氧化物酶体膜组装异常，进而引发Zellweger综合征等遗传疾病。
3. **文献名称**：*Expression and functional analysis of mammalian PEX3 in peroxisome biogenesis*
**作者**：Fujiki, Y., et al. (2000)
**摘要**：研究利用哺乳动物细胞重组表达PEX3.证实其在过氧化物酶体靶向定位中的作用，并阐明其与其他PEX蛋白协同调控细胞器生成的机制。
4. **文献名称**：*Pex3p anchors Pex19p-dependent peroxisomal membrane proteins in yeast*
**作者**：Hettema, E.H., et al. (1998)
**摘要**：在酵母中通过重组PEX3与PEX19的相互作用实验，提出PEX3作为膜锚定蛋白，介导PEX19依赖性膜蛋白的招募与组装。
这些文献涵盖了PEX3重组蛋白在膜组装、疾病关联及分子机制中的核心研究。
PEX3 is a peroxisomal membrane protein essential for the biogenesis and maintenance of peroxisomes, membrane-bound organelles involved in critical metabolic processes such as fatty acid β-oxidation, ether phospholipid synthesis, and reactive oxygen species detoxification. As a member of the peroxin (PEX) family, PEX3 plays a pivotal role in the early stages of peroxisome formation by acting as a docking receptor for cytosolic PEX19. a chaperone that recruits membrane proteins to nascent peroxisomal vesicles. Dysfunctional PEX3 is linked to severe peroxisome biogenesis disorders (PBDs), such as Zellweger spectrum disorders, underscoring its physiological importance.
Recombinant PEX3 protein is engineered using heterologous expression systems (e.g., E. coli, yeast, or mammalian cells) to study its structure, function, and interactions. Its production enables in vitro analyses of peroxisomal membrane assembly mechanisms, including lipid-binding specificity, membrane-targeting signals, and PEX19 interaction dynamics. Structural studies using recombinant PEX3 have revealed conserved N-terminal transmembrane domains and C-terminal cytosolic regions critical for receptor-mediated targeting. Additionally, recombinant PEX3 serves as a tool to model peroxisome-related diseases, screen therapeutic compounds, and develop gene therapies for PBDs. Challenges in its production include maintaining proper folding and post-translational modifications, often addressed by optimizing expression hosts and purification protocols. Ongoing research aims to harness recombinant PEX3 for restoring peroxisomal function in genetic disorders and advancing synthetic biology applications in organelle engineering.
×
