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Mouse Monoclonal MSI2 Antibody

  • 中文名: MSI2抗体
  • 别    名: MSI2H; MGC3245; FLJ36569; MSI2
货号: IPD30178
Price: ¥1280
数量:
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验证与应用

应用及物种
WB 1/500 - 1/2000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/200 - 1/1000 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 1/10000 Human,Mouse,Rat

产品详情

AliasesMSI2H; MGC3245; FLJ36569; MSI2
Entrez GeneID124540
clone2C11
WB Predicted band size35kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenPurified recombinant fragment of human MSI2 expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是关于MSI2抗体的3篇代表性参考文献示例(内容为模拟概括,仅供参考):

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1. **文献名称**:*Musashi2 (MSI2) regulates cancer stem cell properties and tumorigenesis in colorectal cancer*

**作者**:Kudinov AE et al.

**摘要**:本研究通过免疫组织化学(使用MSI2特异性抗体)分析了结直肠癌组织中MSI2蛋白的表达水平,发现其高表达与癌症干细胞标志物(如CD44和LGR5)显著相关。实验表明,MSI2通过调控Wnt/β-catenin信号通路促进肿瘤生长,提示其作为潜在治疗靶点。

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2. **文献名称**:*MSI2 antibody-based detection reveals its critical role in maintaining leukemia stem cells*

**作者**:Ito T et al.

**摘要**:利用MSI2抗体进行流式细胞术和Western blot分析,作者发现MSI2在急性髓系白血病(AML)干细胞中高表达。敲低MSI2可显著抑制白血病细胞的自我更新能力,表明MSI2抗体在鉴定和治疗白血病干细胞中的潜在应用价值。

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3. **文献名称**:*Development and validation of a monoclonal MSI2 antibody for neural stem cell studies*

**作者**:Chen R et al.

**摘要**:该研究开发了一种高特异性抗MSI2单克隆抗体,并通过免疫荧光和共聚焦显微镜验证其在神经干细胞中的定位。结果表明,MSI2在维持神经前体细胞增殖中起关键作用,抗体可作为研究神经发育和再生医学的重要工具。

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**注意**:以上文献为示例,实际引用时请通过PubMed或Google Scholar核对真实文献信息。

背景信息

The MSI2 antibody targets the Musashi homolog 2 (MSI2) protein, a member of the Musashi family of RNA-binding proteins. MSI2 is primarily recognized for its role in post-transcriptional regulation of gene expression by binding to specific mRNA targets, influencing translation, stability, and localization. It is critical in stem cell maintenance, neurodevelopment, and oncogenesis. In stem cells, MSI2 promotes self-renewal by repressing differentiation-associated mRNAs, such as Numb and p21. Dysregulation of MSI2 is linked to various cancers, including leukemia, glioblastoma, and colorectal cancer, where its overexpression correlates with tumor progression, metastasis, and therapy resistance. MSI2 antibodies are widely used in research to investigate its expression patterns, functional mechanisms, and interactions in both physiological and pathological contexts. These antibodies enable detection via techniques like Western blotting, immunohistochemistry, and immunofluorescence, aiding in biomarker studies and therapeutic target validation. Validation of MSI2 antibodies typically involves knockout controls or siRNA knockdown to confirm specificity. As an onconeural protein, MSI2 continues to be a focus in cancer biology and regenerative medicine research.

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