| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CRLF3 |
| Uniprot No | Q8IUI8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-442aa |
| 氨基酸序列 | MRGAMELEPELLLQEARENVEAAQSYRRELGHRLEGLREARRQIKESASQ TRDVLKQHFNDLKGTLGKLLDERLVTLLQEVDTIEQETIKPLDDCQKLIE HGVNTAEDLVREGEIAMLGGVGEENEKLWSFTKKASHIQLDSLPEVPLLV DVPCLSAQLDDSILNIVKDHIFKHGTVASRPPVQIEELIEKPGGIIVRWC KVDDDFTAQDYRLQFRKCTSNHFEDVYVGSETEFIVLHIDPNVDYQFRVC ARGDGRQEWSPWSVPQIGHSTLVPHEWTAGFEGYSLSSRRNIALRNDSES SGVLYSRAPTYFCGQTLTFRVETVGQPDRRDSIGVCAEKQDGYDSLQRDQ AVCISTNGAVFVNGKEMTNQLPAVTSGSTVTFDIEAVTLGTTSNNEGGHF KLRVTISSNNREVVFDWLLDQSCGSLYFGCSFFYPGWKVLVF |
| 预测分子量 | 49,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CRLF3重组蛋白的模拟参考文献示例(非真实文献，仅供格式参考)：
1. **文献名称**：*CRLF3重组蛋白在炎症信号通路中的功能研究*
**作者**：Zhang L, et al.
**摘要**：本研究通过原核系统表达并纯化CRLF3胞外域重组蛋白，发现其能够通过调控JAK-STAT通路抑制巨噬细胞的过度炎症反应，提示CRLF3可能作为炎症性疾病的潜在治疗靶点。
2. **文献名称**：*CRLF3重组蛋白的结构解析及其配体结合特性*
**作者**：Smith J, et al.
**摘要**：利用X射线晶体学解析了CRLF3重组蛋白的胞外结构域三维结构，揭示了其与IL-6家族细胞因子的结合位点，为开发靶向CRLF3的抗体药物提供了结构基础。
3. **文献名称**：*CRLF3重组蛋白在神经退行性疾病中的保护作用*
**作者**：Wang Y, et al.
**摘要**：实验表明，CRLF3重组蛋白可通过激活PI3K/AKT通路减少神经元凋亡，改善阿尔茨海默病模型小鼠的认知功能，提示其神经保护潜力。
4. **文献名称**：*CRLF3重组蛋白的高效表达及纯化工艺优化*
**作者**：Kim H, et al.
**摘要**：通过优化大肠杆菌表达系统，实现了CRLF3重组蛋白的高产量可溶性表达，并建立了两步层析纯化流程，为后续功能研究提供可靠蛋白来源。
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**注**：以上内容为模拟生成，实际文献需通过PubMed、Web of Science等平台检索关键词“CRLF3 recombinant protein”或“CRLF3 function”获取。
CRLF3 (Cytokine Receptor-Like Factor 3), also known as Cytokine Receptor Common Subunit Gamma-Like, is a transmembrane protein belonging to the type I cytokine receptor family. It shares structural homology with the common gamma chain (γc) of interleukin receptors, featuring conserved extracellular cysteine residues and a WSXWS motif critical for ligand binding and receptor activation. Unlike typical cytokine receptors, CRLF3 lacks a canonical intracellular signaling domain, suggesting it may function as a co-receptor or modulator in signaling complexes.
Originally identified through genomic studies, CRLF3 is ubiquitously expressed, with higher levels in immune cells, the brain, and endocrine tissues. Its biological role remains incompletely understood, but emerging evidence links it to cellular stress responses, immune regulation, and neuronal survival. Studies indicate CRLF3 interacts with thrombopoietin (TPO) and erythropoietin (EPO) receptors, potentially fine-tuning JAK-STAT or PI3K-AKT signaling pathways. It may also participate in iron metabolism and apoptosis regulation through unclear mechanisms.
Recombinant CRLF3 proteins are engineered to study its structure-function relationships and therapeutic potential. Typically produced in mammalian or insect cell systems, these proteins retain post-translational modifications for functional authenticity. Purified CRLF3 extracellular domains serve as decoy receptors to probe ligand interactions, while full-length variants aid in elucidating membrane-proximal signaling events. In disease contexts, CRLF3 dysregulation has been observed in neuroinflammatory conditions and certain cancers, making recombinant tools valuable for drug screening and biomarker development. Current research focuses on clarifying its ligand specificity and role in cross-talk between cytokine networks and stress-adaptive pathways.
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