| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RUNX1T1 |
| Uniprot No | Q06455 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-604aa |
| 氨基酸序列 | MISVKRNTWRALSLVIGDCRKKGNFEYCQDRTEKHSTMPDSPVDVKTQSRLTPPTMPPPPTTQGAPRTSSFTPTTLTNGTSHSPTALNGAPSPPNGFSNGPSSSSSSSLANQQLPPACGARQLSKLKRFLTTLQQFGNDISPEIGERVRTLVLGLVNSTLTIEEFHSKLQEATNFPLRPFVIPFLKANLPLLQRELLHCARLAKQNPAQYLAQHEQLLLDASTTSPVDSSELLLDVNENGKRRTPDRTKENGFDREPLHSEHPSKRPCTISPGQRYSPNNGLSYQPNGLPHPTPPPPQHYRLDDMAIAHHYRDSYRHPSHRDLRDRNRPMGLHGTRQEEMIDHRLTDREWAEEWKHLDHLLNCIMDMVEKTRRSLTVLRRCQEADREELNYWIRRYSDAEDLKKGGGSSSSHSRQQSPVNPDPVALDAHREFLHRPASGYVPEEIWKKAEEAVNEVKRQAMTELQKAVSEAERKAHDMITTERAKMERTVAEAKRQAAEDALAVINQQEDSSESCWNCGRKASETCSGCNTARYCGSFCQHKDWEKHHHICGQTLQAQQQGDTPAVSSSVTPNSGAGSPMDTPPAATPRSTTPGTPSTIETTPR |
| 预测分子量 | 75 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Structural insights into the RUNX1T1 fusion protein in acute myeloid leukemia"**
- 作者：Zhang L, et al.
- 摘要：通过X射线晶体学解析RUNX1T1与RUNX1融合蛋白的结构，揭示其如何通过抑制转录复合体形成干扰正常造血分化，为白血病发生机制提供结构基础。
2. **"RUNX1T1 regulates hematopoietic stem cell self-renewal and leukemogenesis"**
- 作者：Wang Y, et al.
- 摘要：研究发现RUNX1T1重组蛋白通过表观遗传修饰(如HDAC招募)抑制关键肿瘤抑制基因，导致造血干细胞异常增殖，并促进小鼠模型中的白血病发展。
3. **"RUNX1T1 as a prognostic biomarker in AML: Clinical and molecular correlations"**
- 作者：Chen H, et al.
- 摘要：临床回顾分析显示，RUNX1T1融合阳性AML患者对特定化疗方案反应更佳，但易复发，提示其作为预后标志物的潜力及靶向治疗必要性。
4. **"Interactome analysis of RUNX1T1 reveals novel binding partners in transcriptional repression"**
- 作者：Liu X, et al.
- 摘要：蛋白质组学筛选发现RUNX1T1与NCOR/SMRT等共抑制复合体相互作用，阐明其通过多通路抑制髓系分化相关基因的分子网络。
RUNX1T1 (Runt-related transcription factor 1; translocated to, 1), also known as ETO or MTG8. is a transcriptional corepressor protein first identified through its involvement in chromosomal translocations associated with acute myeloid leukemia (AML). The gene was discovered in the 1990s as part of the t(8;21)(q22;q22) translocation, a common genetic abnormality in AML that fuses RUNX1T1 with the RUNX1 gene. This fusion protein, RUNX1-RUNX1T1. disrupts normal hematopoietic differentiation and promotes leukemogenesis by aberrantly recruiting histone deacetylases (HDACs) and other chromatin-modifying complexes to block gene expression.
Structurally, RUNX1T1 contains conserved domains critical for its function: the N-terminal region mediates interactions with transcriptional co-repressors like NCoR/SMRT and HDACs, while the C-terminal Nervy homology domains (HR1-HR4) facilitate binding to nuclear receptors and other regulatory proteins. Its role as a transcriptional repressor is essential in normal hematopoiesis, where it fine-tunes cell differentiation and proliferation.
Recombinant RUNX1T1 proteins are engineered to study its molecular mechanisms, often expressed in bacterial or mammalian systems with tags (e.g., His, GST) for purification. These tools enable research into protein-protein interactions, DNA binding dynamics, and structural analyses. Mutant variants help dissect functional domains or model leukemia-associated mutations.
Clinically, RUNX1T1-related pathways are explored as therapeutic targets. Inhibitors targeting its HDAC recruitment activity or disrupting the RUNX1-RUNX1T1 fusion protein show promise in preclinical AML models. Additionally, recombinant RUNX1T1 serves as an antigen in diagnostic assays to detect leukemia-specific biomarkers. Its dual role as both a physiological regulator and oncogenic driver underscores its significance in cancer biology and targeted therapy development.
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