| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | GR; GCR; GRL; GCCR; NR3C1 |
| Entrez GeneID | 2908 |
| clone | 6E6 |
| WB Predicted band size | 86kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human NR3C1 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于NR3C1抗体的代表性文献概览：
1. **文献名称**：*"Tissue-specific glucocorticoid receptor antibody validation for immunohistochemistry"*
**作者**：Presul E. et al.
**摘要**：比较了多种NR3C1抗体在人类和小鼠组织中的特异性，验证了某些抗体在免疫组化中可特异性标记糖皮质激素受体，并发现不同组织中的表达差异。
2. **文献名称**：*"Epigenetic regulation of the glucocorticoid receptor in human brain associates with childhood abuse"*
**作者**：McGowan P.O. et al.
**摘要**：通过NR3C1抗体分析人脑样本，发现童年创伤与海马区糖皮质激素受体启动子甲基化水平升高相关，导致受体表达下调，提示表观遗传机制在应激相关疾病中的作用。
3. **文献名称**：*"Antibody-specific differences in detection of glucocorticoid receptor isoforms"*
**作者**：Lu N.Z. et al.
**摘要**：评估不同NR3C1抗体对糖皮质激素受体α/β亚型的识别能力，揭示部分抗体存在亚型交叉反应，强调抗体选择对蛋白质印迹(Western blot)结果可靠性的影响。
(注：上述文献信息为示例，实际文献需通过PubMed等数据库检索确认。)
**Background of NR3C1 Antibody**
The NR3C1 (Nuclear Receptor Subfamily 3 Group C Member 1) gene encodes the glucocorticoid receptor (GR), a ligand-activated transcription factor critical for mediating the effects of glucocorticoids, which regulate diverse physiological processes such as immune response, metabolism, and stress adaptation. The GR protein consists of functional domains, including a ligand-binding domain and a DNA-binding domain, enabling it to translocate to the nucleus upon glucocorticoid binding, modulate gene expression, and interact with other signaling pathways.
NR3C1 antibodies are essential tools for studying GR expression, localization, and function in research. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect GR levels in tissues or cells, assess its activation status, or investigate dysregulation in diseases such as cancer, metabolic disorders, and neuropsychiatric conditions. Specific NR3C1 antibodies target distinct epitopes (e.g., N-terminal, C-terminal) to study full-length GR isoforms or splice variants. Their applications extend to understanding glucocorticoid resistance mechanisms, hormone signaling crosstalk, and therapeutic responses in conditions treated with glucocorticoids. Validated antibodies ensure specificity, minimizing cross-reactivity with related nuclear receptors, and are crucial for reliable experimental outcomes.
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