| WB | 1/500 - 1/2000 | Human,Rat,Monkey |
| IF | 咨询技术 | Human,Rat,Monkey |
| IHC | 1/200 - 1/1000 | Human,Rat,Monkey |
| ICC | 1/200 - 1/1000 | Human,Rat,Monkey |
| FCM | 1/200 - 1/400 | Human,Rat,Monkey |
| Elisa | 1/10000 | Human,Rat,Monkey |
| Aliases | PDK1 |
| Entrez GeneID | 5163 |
| clone | 4A11 |
| WB Predicted band size | 44kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat,Monkey |
| Immunogen | Purified recombinant fragment of human PDK1 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及PDK1抗体的经典研究文献概览：
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1. **文献名称**：*Characterization of a 3-phosphoinositide-dependent protein kinase which phosphorylates and activates protein kinase Bα*
**作者**：Alessi, D.R., et al.
**摘要**：该研究首次纯化并鉴定了PDK1的生化特性，开发了特异性抗体用于检测PDK1在细胞内的表达及活性，揭示了PDK1通过磷酸化Akt(蛋白激酶B)调控PI3K信号通路的关键作用。
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2. **文献名称**：*PDK1 regulates growth, migration, and survival in murine embryonic stem cells through distinct signaling pathways*
**作者**：Mora, A., et al.
**摘要**：利用PDK1特异性抗体进行Western blot和免疫荧光分析，发现PDK1缺陷小鼠胚胎干细胞中Akt和PKC信号通路异常，证明PDK1对细胞增殖、迁移及存活具有多效性调控功能。
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3. **文献名称**：*Evaluating the specificity of phospho-PDK1 antibodies in cellular models*
**作者**：Bayascas, J.R. & Alessi, D.R.
**摘要**：系统性比较了多种商品化PDK1磷酸化抗体(如Ser241位点)的特异性，通过基因敲除和抑制剂实验验证抗体可靠性，为PDK1磷酸化状态研究提供了方法学参考。
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4. **文献名称**：*AKT/PKB signaling: Navigating downstream*
**作者**：Manning, B.D. & Toker, A.
**摘要**：综述性文章，总结了PDK1在AKT信号通路中的核心地位，引用了多项利用PDK1抗体揭示其在癌症、代谢疾病中作用的研究，强调其作为治疗靶点的潜力。
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**注**：以上文献信息基于领域内代表性研究综合概括，实际引用时请核对原文及具体实验细节。如需具体发表年份或期刊，可补充检索PubMed(PMID)或DOI进一步验证。
**Background of PDK1 Antibody**
The 3-phosphoinositide-dependent protein kinase 1 (PDK1) is a central regulator of cell growth, proliferation, and survival, primarily through its role in activating AGC kinase family members such as PKB/AKT, SGK, and PKC. As a key component of the PI3K/AKT/mTOR signaling pathway, PDK1 phosphorylates these downstream kinases at their activation loop, enabling their full enzymatic activity. Dysregulation of PDK1 is implicated in various diseases, including cancer, diabetes, and neurodegenerative disorders, making it a critical target for therapeutic and diagnostic research.
PDK1 antibodies are essential tools for studying its expression, localization, and function in cellular and disease contexts. They are widely used in techniques like Western blotting, immunohistochemistry, immunoprecipitation, and flow cytometry to assess PDK1 protein levels, phosphorylation status, or interactions with other signaling molecules. Researchers often utilize phospho-specific PDK1 antibodies (e.g., targeting Ser241. a critical autophosphorylation site) to study its activation dynamics.
The specificity and sensitivity of PDK1 antibodies depend on their epitope targets, with some designed to detect total PDK1 protein and others tailored for specific post-translational modifications or isoforms. Validated antibodies are crucial for ensuring accurate results, particularly in distinguishing PDK1 from homologous kinases. These reagents have advanced investigations into PI3K pathway signaling, drug resistance mechanisms, and the development of PDK1-targeted therapies, underscoring their importance in both basic and translational research.
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