| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Pcp4 |
| Uniprot No | P48539 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-62aa |
| 氨基酸序列 | MSERQGAGAT NGKDKTSGEN DGQKKVQEEF DIDMDAPETE RAAVAIQSQF RKFQKKKAGS QS |
| 预测分子量 | 6,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Pcp4重组蛋白的3篇文献概览(注：文献信息为模拟示例，具体研究需查阅真实数据库)：
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1. **文献名称**：*Expression and functional characterization of recombinant Pcp4: A cerebellar calcium sensor protein*
**作者**：Slemmon JR, et al.
**摘要**：该研究通过大肠杆菌系统成功表达并纯化了重组Pcp4蛋白，验证其钙离子结合能力，并揭示其通过调控钙信号通路影响神经元突触可塑性，为研究小脑功能提供了分子基础。
2. **文献名称**：*Pcp4 interacts with Bcl-2 in cancer cells and modulates apoptosis through recombinant protein analysis*
**作者**：Bähr SM, et al.
**摘要**：利用重组Pcp4蛋白进行体外结合实验，发现其与抗凋亡蛋白Bcl-2直接互作，抑制肿瘤细胞凋亡，提示Pcp4可能在前列腺癌等癌症中发挥促生存作用。
3. **文献名称**：*Structural insights into Pcp4-ligand interactions using NMR and recombinant protein technology*
**作者**：Usui N, et al.
**摘要**：通过核磁共振技术解析重组Pcp4的三维结构，鉴定其与特定神经肽结合的活性位点，为开发靶向Pcp4的神经退行性疾病治疗策略提供结构依据。
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建议通过PubMed或Web of Science以“Pcp4 recombinant protein”为关键词检索最新文献，获取更准确的实验数据与应用方向。
**Background of Pcp4 Recombinant Protein**
Pcp4 (Purkinje cell protein 4), also known as PEP-19. is a small, intrinsically disordered protein belonging to the EF-hand calcium-binding protein family. It is highly expressed in the brain, particularly in cerebellar Purkinje cells, and plays roles in modulating calcium signaling, neuronal differentiation, and synaptic plasticity. Its structure includes an N-terminal EF-hand domain that binds calcium ions and a C-terminal region involved in protein-protein interactions. Dysregulation of Pcp4 has been linked to neurological disorders such as Alzheimer’s disease, Huntington’s disease, and spinocerebellar ataxia, as well as certain cancers, highlighting its importance in cellular homeostasis and disease pathways.
Recombinant Pcp4 protein is produced using genetic engineering techniques, often in bacterial (e.g., *E. coli*) or mammalian expression systems (e.g., HEK293 cells). This approach ensures high purity, scalability, and control over post-translational modifications, enabling detailed functional studies. Researchers utilize recombinant Pcp4 to investigate its interactions with calcium-dependent enzymes (e.g., calmodulin), its role in regulating apoptosis, and its potential as a biomarker or therapeutic target.
In neurodegenerative disease research, recombinant Pcp4 aids in elucidating mechanisms of calcium dysregulation and neuronal death. In cancer, it serves as a tool to study its oncogenic or tumor-suppressive roles, depending on tissue context. Additionally, structural analyses of recombinant Pcp4 provide insights into its dynamic conformational changes upon calcium binding, which are critical for its regulatory functions. Overall, recombinant Pcp4 is a vital resource for advancing both basic and translational studies in neuroscience and oncology.
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