| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | PLZF; ZNF145; ZBTB16 |
| Entrez GeneID | 7704 |
| clone | 5B3 |
| WB Predicted band size | 74kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human ZBTB16 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是3篇关于ZBTB16(PLZF)抗体的参考文献及简要摘要：
1. **"The role of PLZF in acute promyelocytic leukemia pathogenesis"**
*作者：Chen et al. (2008)*
摘要：研究使用ZBTB16抗体通过免疫沉淀和Western blot验证PLZF蛋白在白血病细胞中的异常表达，发现其与RARα融合蛋白的相互作用促进白血病发生。
2. **"PLZF regulates germ cell development in mice"**
*作者：Costoya et al. (2004)*
摘要：通过ZBTB16抗体的免疫组化分析，揭示PLZF在小鼠睾丸精原干细胞中的特异性表达，证实其对精子发生和干细胞自我更新的调控作用。
3. **"ZBTB16 mediates transcriptional repression in innate lymphoid cells"**
*作者：Constantinides et al. (2015)*
摘要：利用ZBTB16抗体进行流式细胞术和ChIP-seq实验，发现PLZF在先天性淋巴细胞分化中通过抑制特定基因维持细胞身份。
4. **"PLZF directs the effector program of NKT cells"**
*作者：Kovalovsky et al. (2008)*
摘要：研究使用ZBTB16抗体结合基因敲除模型，证明PLZF控制自然杀伤T细胞(NKT)的功能分化和细胞因子分泌。
The ZBTB16 antibody is a crucial tool for studying the zinc finger and BTB domain-containing protein 16 (ZBTB16), also known as promyelocytic leukemia zinc finger (PLZF). ZBTB16 is a transcription factor involved in cell differentiation, proliferation, and apoptosis. It contains an N-terminal BTB domain for protein interactions and C-terminal zinc finger motifs for DNA binding. Initially identified through its association with acute promyelocytic leukemia (APL), ZBTB16 forms a fusion gene with RARA in rare APL cases due to chromosomal translocations (t(11;17)), impacting myeloid differentiation. Beyond oncology, ZBTB16 regulates stem cell maintenance, immune cell development (e.g., natural killer T cells), and organogenesis (e.g., limb, brain).
ZBTB16 antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and chromatin immunoprecipitation (ChIP) to detect protein expression, localization, and DNA-binding activity. These antibodies aid in exploring ZBTB16's role in transcriptional repression, often mediated by recruiting chromatin-modifying complexes like Polycomb repressors. Commercial ZBTB16 antibodies are typically raised in rabbits or mice, with clones validated for specificity using knockout controls. Research applications span cancer biology, immunology, and developmental studies, particularly in contexts where ZBTB16 acts as a tumor suppressor or oncogenic driver depending on cellular context. Proper validation remains essential due to potential cross-reactivity with related BTB-zinc finger proteins.
×
