Mouse Monoclonal CDC25C Antibody

CDC25C is a dual-specificity phosphatase belonging to the CDC25 family, which plays a critical role in regulating cell cycle progression. It primarily controls the G2/M checkpoint by dephosphorylating and activating cyclin-dependent kinase 1 (CDK1)/cyclin B complexes, thereby driving the transition from G2 phase to mitosis. Dysregulation of CDC25C is implicated in various cancers, as its overexpression can lead to uncontrolled cell proliferation and genomic instability. Antibodies targeting CDC25C are essential tools for studying its expression, localization, and post-translational modifications (e.g., phosphorylation) in response to DNA damage or checkpoint activation. These antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate cell cycle dynamics, DNA repair mechanisms, and cancer biology. Researchers often validate CDC25C antibodies using knockout cell lines or siRNA-mediated knockdown to ensure specificity. Commercially available CDC25C antibodies may recognize different epitopes (e.g., N-terminal or C-terminal regions) and exhibit varying cross-reactivity across species (human, mouse, rat). Their applications extend to clinical research, where altered CDC25C levels serve as potential biomarkers for cancer prognosis or therapeutic targeting. Proper experimental controls, including phosphorylation-specific antibodies, are recommended to study regulatory mechanisms involving CDC25C in cellular stress responses.