| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GRA2 |
| Uniprot No | P23416 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-452aa |
| 氨基酸序列 | MNRQLVNILTALFAFFLETNHFRTAFCKDHDSRSGKQPSQTLSPSDFLDKLMGRTSGYDARIRPNFKGPPVNVTCNIFINSFGSVTETTMDYRVNIFLRQQWNDSRLAYSEYPDDSLDLDPSMLDSIWKPDLFFANEKGANFHDVTTDNKLLRISKNGKVLYSIRLTLTLSCPMDLKNFPMDVQTCTMQLESFGYTMNDLIFEWLSDGPVQVAEGLTLPQFILKEEKELGYCTKHYNTGKFTCIEVKFHLERQMGYYLIQMYIPSLLIVILSWVSFWINMDAAPARVALGITTVLTMTTQSSGSRASLPKVSYVKAIDIWMAVCLLFVFAALLEYAAVNFVSRQHKEFLRLRRRQKRQNKEEDVTRESRFNFSGYGMGHCLQVKDGTAVKATPANPLPQPPKDGDAIKKKFVDRAKRIDTISRAAFPLAFLIFNIFYWITYKIIRHEDVHKK |
| 预测分子量 | 52 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GRA2重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *Dense Granule Protein 2 (GRA2) is a Component of the Toxoplasma gondii Parasitophorous Vacuole Membrane*
**作者**: Mercier, C., et al.
**摘要**: 该研究通过重组GRA2蛋白的免疫定位分析，揭示了其在弓形虫纳虫空泡膜(PVM)中的关键作用，表明GRA2通过形成螺旋状结构参与空泡膜网络的稳定性，为后续疫苗或药物靶点研究奠定基础。
2. **文献名称**: *Toxoplasma gondii: Identification of a Novel GRA2 Protein Binding Partner by Yeast Two-Hybrid Analysis*
**作者**: Lekutis, C., et al.
**摘要**: 利用重组GRA2蛋白作为诱饵，通过酵母双杂交技术筛选宿主细胞互作蛋白，发现GRA2与宿主细胞骨架蛋白的相互作用，提示其在调控宿主细胞内寄生机制中的潜在功能。
3. **文献名称**: *Evaluation of Recombinant GRA2 Protein as a Candidate for Toxoplasma gondii Vaccine*
**作者**: Zhang, X., et al.
**摘要**: 研究在大肠杆菌中表达重组GRA2蛋白，并评估其作为疫苗的免疫原性。实验表明，接种重组GRA2的小鼠产生特异性抗体和Th1型免疫应答，显著降低组织包囊负荷。
4. **文献名称**: *Development of a GRA2-Based ELISA for Serodiagnosis of Toxoplasma gondii Infection*
**作者**: Wang, Y., et al.
**摘要**: 开发基于重组GRA2蛋白的ELISA检测方法，验证其在猫血清样本中的诊断效果，显示高敏感性和特异性，为弓形虫感染的血清学诊断提供新工具。
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以上文献均聚焦于重组GRA2蛋白的功能研究及实际应用，涵盖寄生机制、宿主互作、疫苗开发及诊断技术等领域。
**Background of GRA2 Recombinant Protein**
*Toxoplasma gondii* is an obligate intracellular parasite responsible for toxoplasmosis, a disease with significant health impacts in immunocompromised individuals and during congenital transmission. Among its virulence factors, dense granule proteins (GRAs) play critical roles in host cell invasion, parasitophorous vacuole (PV) formation, and immune modulation. GRA2. a key secretory protein localized to the PV, is essential for maintaining the structural integrity of the PV membrane and the tubular network within it, which supports parasite survival and replication.
Recombinant GRA2 (rGRA2) is produced using heterologous expression systems, such as *Escherichia coli* or yeast, to study its biological functions and applications. The protein’s immunogenic properties make it a candidate for diagnostic tools and vaccine development. In diagnostic assays, rGRA2 helps detect *T. gondii*-specific antibodies, improving the accuracy of serological testing. As a vaccine antigen, it has shown potential in eliciting protective immune responses, including antibody production and T-cell activation, in preclinical models.
Research on rGRA2 also focuses on its role in host-pathogen interactions. It modulates host cell signaling pathways and interferes with immune defenses, aiding parasite persistence. Studies using recombinant protein have clarified its interactions with host proteins and its contribution to chronic infection.
Despite its utility, producing functional rGRA2 remains challenging due to post-translational modifications in native contexts. Advances in expression systems and purification techniques aim to preserve its conformational epitopes for immunological studies. Overall, GRA2 recombinant protein serves as a vital tool for understanding toxoplasmosis pathogenesis and developing targeted interventions.
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