| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | HSP21 |
| Uniprot No | Q5AHH4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-891aa |
| 氨基酸序列 | MSWFGFFDPDFDDFFGRPRKYATEVPPNFNPRKIAQGDNGKGQQVSRYGAGAGHPHRALARRDDFFDDFWKNFSSGKYFVGFDDNVKTTEESDKYVVSYDQENLSPDEVNVDFDKQENELIITVTQETEKDGTKKSSTFHSNLKFEKPVNFDDISAEIGEQGVQVTLPKVHADHEKIVNIPISKAAAKK |
| 预测分子量 | 21,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HSP21重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*Recombinant HSP21 of Arabidopsis thaliana plays a key role in thermotolerance by regulating protein stability*
**作者**：Zhong L., Zhou W., Wang H.
**摘要**：研究报道了拟南芥HSP21重组蛋白在耐热性中的作用，发现其通过稳定叶绿体蛋白复合体，保护光合系统II在高温胁迫下的功能，并揭示了其分子伴侣活性机制。
2. **文献名称**：*Expression and functional analysis of recombinant tomato HSP21 in abiotic stress responses*
**作者**：Chen Y., Li M., Zhang R.
**摘要**：通过在大肠杆菌中表达番茄HSP21重组蛋白，发现其能显著提高转基因植株的耐盐性和抗氧化能力，表明其在植物非生物胁迫应答中具有保护作用。
3. **文献名称**：*Purification and characterization of human HSP21: Implications for neurodegenerative disease models*
**作者**：Smith J., Kumar S., Lee T.
**摘要**：该研究优化了人源HSP21重组蛋白的纯化流程，并证明其在体外抑制α-突触核蛋白异常聚集，提示其可能作为神经退行性疾病的潜在治疗靶点。
(注：以上文献为示例，实际引用需以具体数据库检索结果为准。)
**Background of HSP21 Recombinant Protein**
Heat shock protein 21 (HSP21) is a member of the small heat shock protein (sHSP) family, a class of molecular chaperones crucial for cellular stress responses. These proteins are evolutionarily conserved and play key roles in maintaining protein homeostasis, particularly under stress conditions such as high temperatures, oxidative stress, or pathogen attacks. HSP21 is primarily studied in plants, where it localizes to chloroplasts and contributes to thermotolerance by stabilizing photosynthetic machinery and preventing protein aggregation during heat stress.
The recombinant HSP21 protein is produced using biotechnological methods, such as expression in *E. coli* or yeast systems, followed by purification. This engineered form retains the functional properties of native HSP21. including its ability to bind misfolded proteins and assist in their refolding. Its recombinant production enables large-scale studies on its structure, chaperone mechanisms, and interactions with client proteins.
Research on HSP21 has agricultural and biotechnological implications. In crops like tomatoes (*Solanum lycopersicum*) and *Arabidopsis thaliana*, HSP21 overexpression enhances heat tolerance, suggesting potential applications in developing climate-resilient crops. Beyond plants, HSP21 homologs or analogs in other organisms are explored for their roles in human diseases, such as cancer or neurodegenerative disorders, where protein misfolding is a hallmark.
Overall, HSP21 recombinant protein serves as a valuable tool for unraveling stress adaptation mechanisms and advancing strategies in crop improvement and therapeutic development.
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