| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GCN5L2 |
| Uniprot No | Q92830 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 323-837aa |
| 氨基酸序列 | RSIFTVTRRQLLEKFRVEKDKLVPEKRTLILTHFPKFLSMLEEEIYGANS PIWESGFTMPPSEGTQLVPRPASVSAAVVPSTPIFSPSMGGGSNSSLSLD SAGAEPMPGEKRTLPENLTLEDAKRLRVMGDIPMELVNEVMLTITDPAAM LGPETSLLSANAARDETARLEERRGIIEFHVIGNSLTPKANRRVLLWLVG LQNVFSHQLPRMPKEYIARLVFDPKHKTLALIKDGRVIGGICFRMFPTQG FTEIVFCAVTSNEQVKGYGTHLMNHLKEYHIKHNILYFLTYADEYAIGYF KKQGFSKDIKVPKSRYLGYIKDYEGATLMECELNPRIPYTELSHIIKKQK EIIKKLIERKQAQIRKVYPGLSCFKEGVRQIPVESVPGIRETGWKPLGKE KGKELKDPDQLYTTLKNLLAQIKSHPSAWPFMEPVKKSEAPDYYEVIRFP IDLKTMTERLRSRYYVTRKLFVADLQRVIANCREYNPPDSEYCRCASALE KFFYFKLKEGGLIDK |
| 预测分子量 | 82 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GCN5L2重组蛋白的假设性参考文献示例(若需真实文献，建议通过学术数据库进一步验证)：
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1. **文献名称**：*GCN5L2 modulates mitochondrial acetylation and oxidative metabolism*
**作者**：Scott I. et al.
**摘要**：本研究利用重组GCN5L2蛋白，揭示了其在线粒体蛋白乙酰化中的关键作用。通过体外酶活实验，证明GCN5L2作为乙酰转移酶调控能量代谢通路，影响细胞氧化磷酸化功能。
2. **文献名称**：*Recombinant GCN5L2 purification and its role in cardiac hypertrophy*
**作者**：Wang Y. et al.
**摘要**：作者通过大肠杆菌系统表达并纯化重组GCN5L2蛋白，发现其与心肌肥厚相关转录因子相互作用，乙酰化组蛋白H3K9.促进病理性心脏重塑。
3. **文献名称**：*Structural insights into GCN5L2 catalytic mechanism*
**作者**：Huynh T.K. et al.
**摘要**：通过X射线晶体学解析重组GCN5L2蛋白的三维结构，阐明其底物结合域和乙酰辅酶A结合位点，为设计特异性抑制剂提供结构基础。
4. **文献名称**：*GCN5L2 links acetyl-CoA flux to metabolic disease*
**作者**：Zhang L. et al.
**摘要**：利用重组GCN5L2蛋白进行体外乙酰化分析，发现其响应细胞乙酰辅酶A水平变化，调控代谢相关基因表达，与肥胖和胰岛素抵抗密切相关。
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**注意**：以上文献为示例，实际研究中可能存在名称差异(如GCN5L2或KAT2家族成员)。建议通过PubMed或Google Scholar搜索“GCN5L2 recombinant protein”“GCN5L2 acetylation”等关键词获取真实文献。
**Background of GCN5L2 Recombinant Protein**
GCN5L2. also known as KAT2A (lysine acetyltransferase 2A), is a member of the GNAT (GCN5-related N-acetyltransferase) family of histone acetyltransferases (HATs). It plays a critical role in epigenetic regulation by catalyzing the acetylation of lysine residues on histone proteins, particularly H3K9 and H3K14. thereby modulating chromatin structure and gene expression. This enzyme is highly conserved across eukaryotes and is involved in diverse cellular processes, including transcriptional activation, cell cycle progression, DNA repair, and differentiation.
GCN5L2 functions as part of multiprotein complexes, such as the ADA2A-containing (ATAC) and STAGA complexes, which recruit it to specific genomic loci. Its activity is tightly regulated through interactions with coactivators, post-translational modifications, and cellular signaling pathways. Dysregulation of GCN5L2 has been implicated in various diseases, including cancers, neurodegenerative disorders, and metabolic syndromes, highlighting its therapeutic potential.
Recombinant GCN5L2 protein is engineered for *in vitro* studies to dissect its enzymatic mechanisms, substrate specificity, and interaction partners. Produced using heterologous expression systems (e.g., *E. coli* or mammalian cells), the purified protein retains functional HAT activity, enabling biochemical assays, structural analyses (e.g., X-ray crystallography), and high-throughput drug screening. Researchers utilize this tool to explore its role in chromatin remodeling, identify inhibitors/activators for therapeutic development, and investigate its crosstalk with other epigenetic regulators, such as histone deacetylases (HDACs).
Overall, GCN5L2 recombinant protein serves as a vital resource for advancing our understanding of epigenetic regulation and its implications in health and disease.
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