Mouse Monoclonal WNT10B Antibody

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     Western blot analysis using WNT10B mouse mAb against Hela cell lysate (1).    

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     Confocal Immunofluorescence analysis of PANC-1 cells using WNT10B mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.    

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     Immunohistochemical analysis of paraffin-embedded human normal stomach (A), normal liver (B), normal kidney (C) and rectum cancer tissues (D) using WNT10B mouse mAb with DAB staining.    

WNT10B, a member of the WNT protein family, is a secreted glycoprotein involved in critical biological processes, including embryonic development, tissue homeostasis, and stem cell maintenance. It activates the canonical Wnt/β-catenin signaling pathway by binding to Frizzled receptors and LRP co-receptors, regulating gene expression linked to cell proliferation, differentiation, and fate determination. WNT10B is particularly notable for its roles in adipogenesis, bone metabolism, and hair follicle development. Dysregulation of WNT10B has been implicated in various pathologies, such as cancers (e.g., breast, colorectal), metabolic disorders, and skeletal abnormalities.

WNT10B antibodies are essential tools for detecting and studying the expression, localization, and function of WNT10B in research and diagnostics. These antibodies, typically monoclonal or polyclonal, are validated for specificity and affinity in applications like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. Researchers use them to investigate WNT10B's involvement in disease mechanisms, such as tumorigenesis or metabolic syndrome, and to explore therapeutic targeting. Commercial WNT10B antibodies often undergo rigorous validation using knockout controls or recombinant proteins to ensure reliability. Their utility extends to both basic research and clinical studies, aiding in biomarker discovery and understanding Wnt signaling pathways' complexity.