| WB | 咨询技术 | Human,Mouse,Rat,Rabbit |
| IF | 咨询技术 | Human,Mouse,Rat,Rabbit |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat,Rabbit |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat,Rabbit |
| FCM | 咨询技术 | Human,Mouse,Rat,Rabbit |
| Elisa | 1/10000 | Human,Mouse,Rat,Rabbit |
| Aliases | Isl-1; ISLET1 |
| Entrez GeneID | 3670 |
| clone | 1H9 |
| WB Predicted band size | 39kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat,Rabbit |
| Immunogen | Purified recombinant fragment of human ISL1 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3-4条与ISL1抗体相关的参考文献及其摘要概括：
1. **文献名称**：*ISL1 regulates pancreatic beta cell function and is repressed in diabetic states*
**作者**：Sun et al.
**摘要**：研究利用ISL1抗体揭示ISL1转录因子对胰岛β细胞胰岛素分泌的调控作用，并发现糖尿病模型中ISL1表达下调与β细胞功能障碍相关。
2. **文献名称**：*ISL1 is required for the development of the cardiac outflow tract*
**作者**：Cai et al.
**摘要**：通过免疫组化(使用ISL1抗体)和基因敲除技术，证明ISL1在小鼠心脏流出道发育中不可或缺，其缺失导致严重心脏畸形。
3. **文献名称**：*ISL1 as a biomarker in neuroendocrine tumors*
**作者**：Du et al.
**摘要**：研究发现ISL1抗体在神经内分泌肿瘤组织中特异性标记阳性，提示ISL1可作为此类肿瘤诊断的潜在分子标志物。
4. **文献名称**：*ISL1 directs embryonic stem cell differentiation into motor neurons*
**作者**：Pfaff et al.
**摘要**：利用ISL1抗体追踪胚胎干细胞分化过程，证实ISL1在运动神经元定向分化中的关键调控作用，为神经再生研究提供依据。
(注：以上文献为示例，实际引用需核对具体论文信息)
The ISL1 antibody is a key tool in developmental biology and disease research, targeting ISL1 (Islet-1), a transcription factor critical for organogenesis. ISL1 belongs to the LIM-homeodomain protein family and plays essential roles in embryonic development, particularly in the pancreas, heart, and nervous system. In the pancreas, ISL1 is vital for the differentiation and maturation of pancreatic islet cells, including insulin-producing β-cells, making it a focus in diabetes research. In cardiac development, it regulates the formation of the heart’s outflow tract, atria, and right ventricle by directing multipotent cardiovascular progenitor cells. Within the nervous system, ISL1 is involved in motor neuron specification and sensory neuron development.
ISL1 antibodies are widely used in techniques like immunohistochemistry, Western blotting, and flow cytometry to detect ISL1 expression patterns in tissues, stem cell models, or disease contexts. These antibodies help elucidate mechanisms underlying congenital defects (e.g., heart malformations), neurodevelopmental disorders, and endocrine dysfunctions. Monoclonal and polyclonal ISL1 antibodies derived from hosts like rabbits or mice are validated for specificity, often through knockout controls. Their applications extend to regenerative medicine, cancer research (e.g., studying ISL1 in tumors), and biomarker discovery, underscoring their versatility in both basic and translational studies.
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