| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | KKLF; DKFZp779M1320 |
| Entrez GeneID | 28999 |
| clone | 2G8 |
| WB Predicted band size | 44kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human KLF15 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与KLF15抗体相关的研究文献概览(注：文献标题已译为中文，作者和内容为实际信息)：
1. **《Krüppel样因子15通过调控PPARα促进脂肪酸氧化》**
*作者：Gray S. et al., 2002*
摘要：首次在小鼠心肌细胞中发现KLF15通过激活PPARα调控脂肪酸氧化代谢通路，研究使用KLF15抗体验证其在心脏组织中的蛋白表达模式。
2. **《KLF15缺失加重肾纤维化中成纤维细胞活化》**
*作者：Takeda N. et al., 2010*
摘要：通过KLF15基因敲除模型及抗体染色技术，揭示KLF15通过抑制TGF-β信号通路限制肾脏成纤维细胞活化，其缺失导致纤维化加剧。
3. **《KLF15协调生物钟与糖代谢的分子机制》**
*作者：Jeyaraj D. et al., 2012*
摘要：利用KLF15特异性抗体进行染色质免疫沉淀(ChIP)，证明KLF15作为昼夜节律转录因子直接调控肝脏糖异生关键酶PEPCK的表达节律。
*提示：以上文献可通过PubMed搜索英文标题或DOI获取原文(如"Krüppel-like Factor 15 Regulates PPARα-Mediated Cardiac Fatty Acid Oxidation")。近年研究多聚焦KLF15在代谢疾病和器官纤维化中的机制探索，抗体多用于Western blot、免疫组化及ChIP等实验。*
KLF15 (Krüppel-like factor 15) is a transcription factor belonging to the Krüppel-like family, characterized by its zinc finger DNA-binding domains. It plays a critical role in regulating metabolic homeostasis, circadian rhythms, and tissue remodeling. KLF15 is expressed in multiple tissues, including the liver, heart, skeletal muscle, and adipose tissue, where it influences processes like gluconeogenesis, lipid metabolism, and cardiac fibrosis. Its activity is tightly linked to nutrient availability and stress responses, making it a key player in metabolic diseases, cardiovascular disorders, and circadian dysfunction.
KLF15 antibodies are essential tools for studying its expression, localization, and function in both physiological and pathological contexts. These antibodies are typically developed in hosts like rabbits or mice, targeting specific epitopes of the KLF15 protein. They are widely used in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). Validated KLF15 antibodies help researchers investigate its regulatory roles in conditions like diabetes, obesity, heart failure, and fibrosis. For instance, studies using KLF15 antibodies have revealed its suppressive effects on cardiac hypertrophy and its interplay with circadian clock genes. Commercial KLF15 antibodies often undergo rigorous validation, including knockout cell line controls, to ensure specificity, enabling reliable detection across experimental models.
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