| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PDCD2 |
| Uniprot No | Q16342 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1–344aa |
| 氨基酸序列 | MAAAGARPVELGFAESAPAWRLRSEQFPSKVGGRPAWLGAAGLPGPQALACELCGRPLSFLLQVYAPLPGRPDAFHRCIFLFCCREQPCCAGLRVFRNQLPRKNDFYSYEPPSENPPPETGESVCLQLKSGAHLCRVCGCLGPKTCSRCHKAYYCSKEHQTLDWRLGHKQACAQPDHLDHIIPDHNFLFPEFEIVIETEDEIMPEVVEKEDYSEIIGSMGEALEEELDSMAKHESREDKIFQKFKTQIALEPEQILRYGRGIAPIWISGENIPQEKDIPDCPCGAKRILEFQVMPQLLNYLKADRLGKSIDWGILAVFTCAESCSLGTGYTEEFVWKQDVTDTP |
| 预测分子量 | 44.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PDCD2重组蛋白的模拟参考文献示例(仅供学术写作格式参考，具体文献需通过学术数据库查询)：
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1. **文献名称**: *"Recombinant PDCD2 Protein Induces Apoptosis in Human Leukemia Cells via Mitochondrial Pathway"*
**作者**: Zhang L, et al.
**摘要**: 研究报道了PDCD2重组蛋白在体外通过激活线粒体途径诱导白血病细胞凋亡，揭示了其潜在的抗肿瘤机制。
2. **文献名称**: *"Expression and Purification of Functional PDCD2 in Prokaryotic System"*
**作者**: Kim S, Park JH.
**摘要**: 成功在大肠杆菌系统中表达并纯化具有生物活性的PDCD2重组蛋白，验证了其与DNA结合的能力及对细胞周期的影响。
3. **文献名称**: *"Structural Insights into PDCD2-DNA Interaction by Cryo-EM Analysis"*
**作者**: Gupta R, et al.
**摘要**: 通过冷冻电镜解析PDCD2重组蛋白与DNA复合物的三维结构，阐明其参与基因转录调控的结构基础。
4. **文献名称**: *"PDCD2 Deficiency Alters Hematopoietic Stem Cell Differentiation via Modulating Wnt/β-catenin Signaling"*
**作者**: Chen X, et al.
**摘要**: 利用重组PDCD2蛋白进行功能回补实验，证明其通过调控Wnt通路影响造血干细胞分化和自我更新能力。
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**说明**：以上文献为模拟示例，实际研究需参考PubMed、Web of Science等平台的权威论文。PDCD2(Programmed Cell Death 2)的研究多聚焦于凋亡调控、癌症及干细胞领域，重组蛋白技术常用于其分子机制探索。
**Background of PDCD2 Recombinant Protein**
Programmed Cell Death 2 (PDCD2) is a conserved nuclear protein implicated in apoptosis, cell cycle regulation, and hematopoiesis. It belongs to the MYND domain-containing protein family, characterized by a zinc finger motif that facilitates interactions with transcriptional regulators or chromatin modifiers. PDCD2 was initially identified for its role in programmed cell death, but subsequent studies revealed broader functions, including modulating hematopoietic stem cell differentiation and maintaining genomic stability.
PDCD2 interacts with diverse molecular partners, such as the tumor suppressor menin and components of the polycomb repressive complex, suggesting involvement in epigenetic regulation. Dysregulation of PDCD2 has been linked to hematological disorders and cancers. For instance, reduced PDCD2 expression correlates with acute myeloid leukemia progression, while its overexpression may inhibit tumor growth in certain contexts, highlighting context-dependent roles.
Recombinant PDCD2 protein is engineered using expression systems like *E. coli* or mammalian cells to study its biochemical and functional properties. The purified protein retains structural and functional integrity, enabling *in vitro* studies on DNA binding, protein-protein interactions, and enzymatic activity. Researchers utilize PDCD2 recombinant protein to investigate its role in apoptosis signaling, cell cycle checkpoints, and hematopoietic development.
Recent applications include screening for PDCD2-targeting therapeutics and developing diagnostic tools for cancers. However, its precise mechanisms remain partially unresolved, necessitating further exploration. The availability of recombinant PDCD2 accelerates mechanistic studies, offering insights into its dual roles in health and disease, with potential implications for precision medicine.
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