| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | pht3 |
| Uniprot No | Q05183 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-439aa |
| 氨基酸序列 | MLTPEENLLLCRVEGDAPMGQMMRRHWTPVCLLEEVSEPDGTPVRARLFGEDLVVFRDTDGRVGVMDEYCPHRRVSLIYGRNENSGLRCLYHGWKMDVDGNVVEMVSEPAASNMCQKVKHTAYKTREWGGFVWAYMGPQDAIPEFVPPAWAPHEHVRVSIAKAIIPCNWAQILEGAIDSAHSSSLHSSDFVPARVGGAEATSKNWLRPSTDKAPRMQVERTSYGFRYAALRRPIQNAATSEYVRSTVFVAPATALIPPNNLYNVANINVPIDDTHTAFYFMAWGNPDNTPETETWRKFLGQQVGIDLDDSYRPLRNDGNRFFQDREAMKNGNFTGIKGFPNQDIAMWVTMGPIADRSDERLGASDLAVVEFRRVMLDALAAFQAGESAIGTGEKAIPSRICSFQAIVSKDIDWRDYQARYVWALDDANIVAEPDYEVHT |
| 预测分子量 | 49,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PHTr3重组蛋白的假设性参考文献示例(注：PHTr3为虚构名称，实际文献需通过数据库验证)：
1. **《重组PHTr3蛋白在大肠杆菌中的高效表达与纯化》**
作者：李明等
摘要：本研究优化了PHTr3基因的密码子，利用大肠杆菌表达系统实现了可溶性重组PHTr3蛋白的高效表达，并通过镍柱亲和层析获得高纯度蛋白，为后续功能研究奠定基础。
2. **《PHTr3蛋白的晶体结构解析及其底物结合机制》**
作者：Chen, X. et al.
摘要：通过X射线晶体学解析了PHTr3蛋白的三维结构，揭示了其活性位点与底物结合的关键氨基酸残基，提出了可能的酶催化机制。
3. **《PHTr3重组蛋白在肿瘤细胞凋亡中的调控作用》**
作者：Wang, Y. & Zhang, L.
摘要：体外实验表明，PHTr3重组蛋白通过激活线粒体凋亡通路显著抑制癌细胞增殖，提示其作为潜在肿瘤治疗靶点的价值。
4. **《PHTr3重组蛋白的稳定性及冻干制剂开发》**
作者：刘强等
摘要：系统评估了PHTr3重组蛋白在不同温度/pH下的稳定性，并成功开发出冻干制剂，显著提高了长期储存的活性保留率。
(提示：以上为模拟文献，实际研究中请通过PubMed/Web of Science等平台以准确关键词检索。)
**Background of Pht3 Recombinant Protein**
Pht3 (Pseudomonas harpin-triggered 3) is a recombinant protein derived from phytopathogenic bacteria, particularly *Pseudomonas syringae*, which infects plants by deploying virulence factors via the type III secretion system (T3SS). Pht3 belongs to a family of effector proteins that manipulate host cellular processes to suppress plant immune responses, facilitating bacterial colonization. Initially identified for its role in eliciting hypersensitive responses (HR) in non-host plants, Pht3 has garnered attention for its dual functionality as both a pathogenicity factor and a potential immune elicitor.
Structurally, Pht3 contains conserved domains associated with bacterial effectors, including motifs for secretion and host-targeting. Its recombinant form is engineered through heterologous expression systems (e.g., *E. coli*), enabling studies on its biochemical properties and interactions with plant proteins. Pht3 is hypothesized to interfere with plant defense mechanisms, such as disrupting hormone signaling (e.g., jasmonic acid and salicylic acid pathways) or modifying host transcription through enzymatic activities.
Research on recombinant Pht3 has implications for understanding plant-microbe interactions and developing biotechnological tools. For instance, it serves as a model to dissect effector-triggered immunity (ETI) and to engineer disease-resistant crops by exploiting its immunogenic epitopes. Additionally, structural analyses of Pht3 aid in designing inhibitors to block pathogenic effectors. Despite progress, the precise molecular mechanisms of Pht3 remain partially unresolved, necessitating further studies to clarify its role in pathogenesis and its potential applications in agriculture and synthetic biology.
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