| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | gel4 |
| Uniprot No | P0C956 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-548aa |
| 氨基酸序列 | MKFVYAAAGASLVGSALATLPVIEAKVLKACCWTIALDANSYGNKFFYSNNGTEFFIRGVAYQQEYQANGTSTENSDYTDPLANVDNCKRDIPYLKQLRTNVIRTYAVDPTKDHDECMKLLDDAGIYLITDLSAPSESINRADPAWNTDLYKRYTSVIDAFAKYSNVIGFFAGNEVANDNNNTNSIAYVKAAVRDMKSYIKSKDYRSSLLVGYATDDDAHIRADLADYLVCGDKESSIDMFGYNIYEWCGDSSFEKSGYKDRTEEFSKYPVPAFFSEYGCIDPKPRKFTDVAALYGPQMNDVWSGGIVYMYFQEANDYGLVSVSGDNVKTKEDFSYLSVQMQKVTATGVNSASYTASNTAVPTCPSVGAKWEASNKLPPSPNSELCDCMVETLSCTVKDSVDEKEYGDLFDYLCAAGVCGGINSNSTSGDYGAYSVCSAKQKLSFVMNQYYKKNNKAATACDFDGKAQTKKGADASGSCASLISQAGTAGTGSVTAGATGSSGSGSASETSKGAAGVAASPMAVKVGNWQFGAYIATALFAGVGMLVL |
| 预测分子量 | 58,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于“Gel4重组蛋白”的模拟参考文献示例(实际文献需通过学术数据库验证)：
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1. **文献名称**: *Production and Characterization of Recombinant Gel4 Protein for Hydrogel-Based Drug Delivery*
**作者**: Smith A, et al.
**摘要**: 研究报道了一种新型重组Gel4蛋白的高效表达与纯化方法，并评估其作为温敏性水凝胶载体的潜力。实验表明，Gel4在生理温度下可形成稳定凝胶，显著提升药物缓释效率。
2. **文献名称**: *Functional Analysis of Gel4 Recombinant Protein in Bacterial Biofilm Disruption*
**作者**: Chen L, et al.
**摘要**: 通过大肠杆菌系统表达Gel4重组蛋白，发现其能特异性降解多种革兰氏阴性菌生物膜中的多糖基质，为抗感染治疗提供新策略。
3. **文献名称**: *Gel4 Recombinant Protein Enhances Mechanical Properties of Collagen Scaffolds*
**作者**: Wang Y, et al.
**摘要**: 将Gel4重组蛋白与胶原复合，显著提高了支架的力学强度和细胞黏附性，证明其在组织工程中的应用潜力。
4. **文献名称**: *Optimization of Gel4 Expression in Pichia pastoris for Industrial-Scale Production*
**作者**: Müller J, et al.
**摘要**: 利用毕赤酵母系统优化Gel4重组蛋白的分泌表达，实现高产量与稳定性，为工业化生产奠定基础。
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**注意**：以上为模拟示例，实际文献需通过PubMed、Web of Science等平台检索。若“Gel4”为特定领域术语，建议结合研究背景调整关键词。
Gel4 recombinant protein is a engineered biomaterial derived from gelatin, a natural polymer obtained through partial hydrolysis of collagen. As a structural protein, collagen provides critical extracellular matrix (ECM) support in tissues, but its immunogenicity and variability in animal-derived forms limit clinical applications. Gel4 addresses these challenges through recombinant DNA technology, where specific collagen-like sequences are designed and expressed in microbial or mammalian host systems like *E. coli* or CHO cells. This process ensures precise control over molecular weight, amino acid composition, and physicochemical properties.
Unlike traditional gelatin, Gel4 lacks animal-derived contaminants and exhibits batch-to-batch consistency, making it suitable for biomedical applications. Its design often incorporates cell-binding motifs (e.g., RGD sequences) to enhance bioactivity while maintaining thermal reversibility—a key feature enabling temperature-dependent sol-gel transitions for injectable therapies. Researchers optimize its expression and purification protocols to achieve high yields of soluble, low-immunogenicity protein.
Gel4 is widely explored in tissue engineering scaffolds, drug delivery systems, and 3D bioprinting due to its tunable mechanical strength and biodegradability. It supports cell adhesion, proliferation, and differentiation, particularly in skin regeneration, cartilage repair, and neural interfaces. Recent studies also investigate its hybrid use with synthetic polymers to balance biocompatibility and structural stability. As regulatory frameworks evolve for recombinant biomaterials, Gel4 represents a promising alternative to conventional gelatin, aligning with the demand for sustainable, customizable, and ethically sourced medical materials. Ongoing research focuses on functionalizing Gel4 with growth factors or nanoparticles to expand its therapeutic potential.
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