| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | CYP74A |
| Uniprot No | Q96242 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 34-518aa |
| 氨基酸序列 | SGSETPDLTVATRTGSKDLPIRNIPGNYGLPIVGPIKDRWDYFYDQGAEEFFKSRIRKYNSTVYRVNMPPGAFIAENPQVVALLDGKSFPVLFDVDKVEKKDLFTGTYMPSTELTGGYRILSYLDPSEPKHEKLKNLLFFLLKSSRNRIFPEFQATYSELFDSLEKELSLKGKADFGGSSDGTAFNFLARAFYGTNPADTKLKADAPGLITKWVLFNLHPLLSIGLPRVIEEPLIHTFSLPPALVKSDYQRLYEFFLESAGEILVEADKLGISREEATHNLLFATCFNTWGGMKILFPNMVKRIGRAGHQVHNRLAEEIRSVIKSNGGELTMGAIEKMELTKSVVYECLRFEPPVTAQYGRAKKDLVIESHDAAFKVKAGEMLYGYQPLATRDPKIFDRADEFVPERFVGEEGEKLLRHVLWSNGPETETPTVGNKQCAGKDFVVLVARLFVIEIFRRYDSFDIEVGTSPLGSSVNFSSLRKASF |
| 预测分子量 | 61.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CYP74A重组蛋白的3篇代表性文献的简要信息：
1. **文献名称**：*Functional characterization of recombinant CYP74A from Arabidopsis thaliana: A key enzyme in jasmonate biosynthesis*
**作者**：Brash AR, et al.
**摘要**：研究报道了拟南芥CYP74A重组蛋白在大肠杆菌中的高效表达与纯化，证实其催化亚麻酸氢过氧化物生成茉莉酸前体，揭示了其在植物胁迫响应中的关键酶活性。
2. **文献名称**：*Substrate specificity and catalytic mechanism of recombinant CYP74A3 from Solanum lycopersicum*
**作者**：Hughes RK, et al.
**摘要**：通过酵母表达系统获得番茄CYP74A3重组蛋白，分析其底物选择性及pH依赖性，证明其优先催化13-氢过氧化亚麻酸生成挥发性醛类化合物，阐明了酶促反应动力学特征。
3. **文献名称**：*Crystal structure of recombinant CYP74A1 reveals insights into allene oxide synthase activity*
**作者**：Li H, et al.
**摘要**：首次解析了重组表达并纯化的CYP74A1蛋白晶体结构，揭示了其活性中心独特的氢键网络与底物结合模式，为设计酶活性调控分子提供了结构基础。
*注：以上为示例性内容，实际文献需根据具体研究补充。建议通过PubMed或Web of Science以"CYP74A recombinant protein"为关键词检索最新研究。*
CYP74A represents a subfamily of cytochrome P450 enzymes found in plants, primarily involved in the oxylipin biosynthesis pathway. Unlike typical P450s that require molecular oxygen and NADPH-dependent redox partners, CYP74A enzymes catalyze isomerization or cleavage reactions using fatty acid hydroperoxides as substrates, functioning as hydroperoxide lyases (HPLs), allene oxide synthases (AOSs), or divinyl ether synthases (DESs). These enzymes play critical roles in plant stress responses, particularly in the production of jasmonates (key signaling molecules for defense and development) and volatile aldehydes (e.g., leaf wound-response compounds).
Recombinant CYP74A proteins are engineered to study their structure-function relationships, substrate specificity, and catalytic mechanisms. Heterologous expression systems (e.g., *E. coli*, yeast) enable large-scale production, overcoming low natural abundance in plant tissues. Studies on recombinant CYP74A have revealed its role in determining product specificity—for instance, subtle amino acid variations can switch enzyme activity between AOS and HPL pathways. Structural analyses (e.g., X-ray crystallography) highlight conserved P450 folds and unique substrate-channel features governing hydroperoxide positioning.
Biotechnological applications include modulating plant stress tolerance or aroma profiles in crops. For example, CYP74A-derived HPLs are exploited to produce green leaf volatiles for flavor/fragrance industries. Challenges remain in optimizing catalytic efficiency and stability for industrial use. Overall, recombinant CYP74A studies bridge plant biochemistry with synthetic biology, offering tools to manipulate oxylipin signaling for agricultural or commercial purposes.
×
