| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LSS |
| Uniprot No | P48449 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-732aa |
| 氨基酸序列 | TEGTCLRRRGGPYKTEPATDLGRWRLNCERGRQTWTYLQDERAGREQTGLEAYALGLDTKNYFKDLPKAHTAFEGALNGMTFYVGLQAEDGHWTGDYGGPLFLLPGLLITCHVARIPLPAGYREEIVRYLRSVQLPDGGWGLHIEDKSTVFGTALNYVSLRILGVGPDDPDLVRARNILHKKGGAVAIPSWGKFWLAVLNVYSWEGLNTLFPEMWLFPDWAPAHPSTLWCHCRQVYLPMSYCYAVRLSAAEDPLVQSLRQELYVEDFASIDWLAQRNNVAPDELYTPHSWLLRVVYALLNLYEHHHSAHLRQRAVQKLYEHIVADDRFTKSISIGPISKTINMLVRWYVDGPASTAFQEHVSRIPDYLWMGLDGMKMQGTNGSQIWDTAFAIQALLEAGGHHRPEFSSCLQKAHEFLRLSQVPDNPPDYQKYYRQMRKGGFSFSTLDCGWIVSDCTAEALKAVLLLQEKCPHVTEHIPRERLCDAVAVLLNMRNPDGGFATYETKRGGHLLELLNPSEVFGDIMIDYTYVECTSAVMQALKYFHKRFPEHRAAEIRETLTQGLEFCRRQQRADGSWEGSWGVCFTYGTWFGLEAFACMGQTYRDGTACAEVSRACDFLLSRQMADGGWGEDFESCEERRYLQSAQSQIHNTCWAMMGLMAVRHPDIEAQERGVRCLLEKQLPNGDWPQENIAGVFNKSCAISYTSYRNIFPIWALGRFSQLYPERALAGHP |
| 预测分子量 | 89.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LSS(Lanosterol Synthase)重组蛋白的3篇参考文献示例(注：文献信息为假设性概括，仅供参考)：
1. **文献名称**：*Heterologous Expression and Characterization of Recombinant Human Lanosterol Synthase*
**作者**：Zhang Y. et al.
**摘要**：本研究成功在大肠杆菌中表达了具有活性的人源LSS重组蛋白，并优化了纯化条件。通过体外酶活实验证实其催化2.3-氧化角鲨烯生成羊毛甾醇的能力，为胆固醇合成通路研究提供工具。
2. **文献名称**：*Crystal Structure of Lanosterol Synthase Reveals Catalytic Mechanism*
**作者**：Wang X. et al.
**摘要**：通过X射线晶体学解析了LSS重组蛋白的三维结构，揭示了其活性位点及底物结合模式，阐明了环化反应的关键氨基酸残基，为靶向抑制剂设计奠定结构基础。
3. **文献名称**：*LSS Overexpression Promotes Tumorigenesis in Hepatocellular Carcinoma*
**作者**：Li H. et al.
**摘要**：利用重组LSS蛋白体外验证其在肝癌细胞中的功能，发现其通过调控胆固醇代谢促进细胞增殖和迁移，提示LSS可能作为潜在治疗靶点。
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**说明**：实际文献需通过学术数据库(如PubMed、Web of Science)检索关键词“Lanosterol Synthase recombinant”获取。若LSS指其他蛋白(如“Lactate Synthase System”)，建议进一步明确缩写全称。
**Background of LSS Recombinant Proteins**
LSS (Lactococcus lactis Secretion Signal) recombinant proteins are engineered proteins produced using secretion signal peptides derived from *Lactococcus lactis*, a Gram-positive bacterium widely utilized in biotechnology. This system leverages the bacterium’s natural protein secretion machinery to efficiently export recombinant proteins into the extracellular environment during fermentation. Unlike cytoplasmic expression systems, secretion-based approaches simplify downstream purification by reducing host cell contaminants, enhancing yield, and minimizing protein aggregation or misfolding.
The LSS system is particularly valued for its compatibility with food-grade microbial hosts, making it suitable for producing proteins intended for therapeutic, diagnostic, or industrial applications. For instance, *L. lactis* is recognized as Generally Regarded as Safe (GRAS), which streamlines regulatory approval for products like vaccines, enzymes, or bioactive peptides. Additionally, the secretion signals can be tailored to optimize protein export, enabling high-level production of complex proteins, including those requiring post-translational modifications when combined with engineered host strains.
Recent advancements in synthetic biology and strain engineering have further improved the LSS platform. Modular vectors, inducible promoters, and CRISPR-based genome editing allow precise control over protein expression and secretion efficiency. Challenges remain, such as scaling production for industrial use and ensuring proper folding of eukaryotic proteins. However, the system’s cost-effectiveness, rapid growth kinetics, and scalability continue to drive its adoption in biomanufacturing.
Overall, LSS recombinant proteins represent a versatile tool for sustainable and scalable protein production, bridging gaps between laboratory research and commercial applications in healthcare, agriculture, and bio-based industries.
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