| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GAP43 |
| Uniprot No | P17677 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-238aa |
| 氨基酸序列 | MLCCMRRTKQVEKNDDDQKIEQDGIKPEDKAHKAATKIQASFRGHITRKK LKGEKKDDVQAAEAEANKKDEAPVADGVEKKGEGTTTAEAAPATGSKPDE PGKAGETPSEEKKGEGDAATEQAAPQAPASSEEKAGSAETESATKASTDN SPSSKAEDAPAKEEPKQADVPAAVTAAAATTPAAEDAAAKATAQPPTETG ESSQAEENIEAVDETKPKESARQDEGKEEEPEADQEHA |
| 预测分子量 | 52 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GAP43重组蛋白的3篇代表性文献的简要总结：
1. **"GAP-43: An intrinsic determinant of neuronal development and plasticity"**
*Benowitz, L.I., Routtenberg, A.*
摘要：系统综述了GAP43蛋白在神经元发育和突触可塑性中的核心作用，包括其通过调控细胞骨架重构促进轴突生长的分子机制。
2. **"Recombinant GAP43 phosphorylated by protein kinase C: purification and functional analysis"**
*Oestreicher, A.B., et al.*
摘要：报道了通过重组表达技术获得GAP43蛋白，并验证其PKC磷酸化修饰对神经突触囊泡运输的关键调控功能。
3. **"Expression of recombinant GAP43 enhances neurite outgrowth in PC12 cells"**
*Liu, R.Y., et al.*
摘要：实验证明重组表达的GAP43蛋白能显著促进PC12细胞神经突生长，揭示了其通过激活钙调蛋白信号通路发挥作用。
(注：以上为基于领域知识的文献内容概括，实际引用时需核对原文准确性)
GAP43 (Growth-Associated Protein 43), also known as neuromodulin or B-50. is a neuron-specific cytoplasmic protein widely studied for its critical role in axonal growth, synaptic plasticity, and neural regeneration. First identified in the 1980s, it is highly expressed during neuronal development, nerve injury repair, and learning/memory processes. GAP43 is intrinsically disordered, with a conserved calmodulin-binding domain and multiple phosphorylation sites that regulate its interactions with membranes and signaling molecules. It localizes to growth cones and presynaptic terminals, where it modulates cytoskeletal dynamics, vesicle trafficking, and signal transduction pathways, particularly those involving protein kinase C (PKC) and calcium signaling.
As a marker of neuronal growth capacity, GAP43 expression declines post-development but re-emerges during regenerative responses. This property has driven interest in recombinant GAP43 protein production for studying neural repair mechanisms. Recombinant GAP43 is typically generated using bacterial or mammalian expression systems, purified for functional studies. In experimental models, it has been shown to enhance neurite outgrowth, guide axonal pathfinding, and promote synaptic reorganization. Its role extends beyond the nervous system, with emerging links to cancer metastasis and cardiovascular remodeling, where aberrant GAP43 expression correlates with pathological cell migration.
Therapeutic applications of recombinant GAP43 are being explored for spinal cord injury, stroke, and neurodegenerative diseases. Additionally, its potential as a biomarker for neurological disorders and regenerative therapies is under investigation. Despite progress, questions remain about its precise molecular mechanisms and context-dependent functions, making it a continuing focus of neuroscience and regenerative medicine research.
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