| 纯度 | > 95 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | AIFM1 |
| Uniprot No | O95831 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 121-613aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMEEVPQDKAPSHVPFLLIGGGTAAFAAARS IRARDPGARVLIVSEDPELPYMRPPLSKELWFSDDPNVTKTLRFKQWNGK ERSIYFQPPSFYVSAQDLPHIENGGVAVLTGKKVVQLDVRDNMVKLNDGS QITYEKCLIATGGTPRSLSAIDRAGAEVKSRTTLFRKIGDFRSLEKISRE VKSITIIGGGFLGSELACALGRKARALGTEVIQLFPEKGNMGKILPEYLS NWTMEKVRREGVKVMPNAIVQSVGVSSGKLLIKLKDGRKVETDHIVAAVG LEPNVELAKTGGLEIDSDFGGFRVNAELQARSNIWVAGDAACFYDIKLGR RRVEHHDHAVVSGRLAGENMTGAAKPYWHQSMFWSDLGPDVGYEAIGLVD SSLPTVGVFAKATAQDNPKSATEQSGTGIRSESETESEASEITIPPSTPA VPQAPVQGEDYGKGVIFYLRDKVVVGIVLWNIFNRMPIARKIIKDGEQHE DLNEVAKLFNIHED |
| 预测分子量 | 67 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AIFM1重组蛋白的3篇示例文献(注：文献为示例性概括，具体内容需参考真实数据库)：
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1. **文献名称**：*Recombinant AIFM1 Protein Expression in E. coli and Its Apoptotic Activity*
**作者**：Li, X. et al.
**摘要**：本研究利用大肠杆菌系统成功表达并纯化了重组AIFM1蛋白，通过体外实验证实其能够诱导 caspase 非依赖的细胞凋亡，为后续功能研究提供了可溶性蛋白工具。
2. **文献名称**：*Structural Insights into AIFM1-Mediated Chromatin Degradation in Mitochondrial Apoptosis*
**作者**：Wang, Y. et al.
**摘要**：通过冷冻电镜解析了重组AIFM1蛋白的三维结构，揭示了其与DNA结合的分子机制，阐明其在凋亡过程中参与染色质断裂的关键结构域。
3. **文献名称**：*AIFM1 Mutations and Neurodegeneration: Functional Analysis Using Recombinant Protein Models*
**作者**：Garcia, S. et al.
**摘要**：构建了携带致病性突变的AIFM1重组蛋白，发现其线粒体定位及促凋亡功能受损，为X-linked Charcot-Marie-Tooth病等神经退行性疾病的机制提供了实验依据。
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如需实际文献，建议通过PubMed或Google Scholar检索关键词“AIFM1 recombinant protein expression”或“AIFM1 structure function”。
AIFM1 (Apoptosis-Inducing Factor Mitochondrial 1) is a flavoprotein located in the mitochondrial intermembrane space, playing dual roles in cellular energy metabolism and apoptosis regulation. Initially identified as a caspase-independent mediator of programmed cell death, AIFM1 is released from mitochondria during apoptosis and translocates to the nucleus, where it triggers chromatin condensation and DNA fragmentation. Beyond its apoptotic function, AIFM1 participates in maintaining mitochondrial structure, redox homeostasis, and the assembly of respiratory chain Complex I.
Recombinant AIFM1 protein is engineered to study its structure-function relationships and therapeutic potential. Produced via heterologous expression systems (e.g., E. coli or mammalian cells), it retains key domains: an N-terminal FAD-binding domain critical for redox activity and a C-terminal NADH-binding domain involved in oligomerization. Post-translational modifications, such as phosphorylation, may influence its dual roles, making recombinant variants valuable for dissecting molecular mechanisms.
Research using recombinant AIFM1 has revealed its context-dependent behavior—promoting cell survival under mild oxidative stress but inducing apoptosis under severe damage. Dysregulation of AIFM1 is linked to neurodegenerative disorders (e.g., Parkinson’s, Charcot-Marie-Tooth disease), cancer, and ischemia-reperfusion injury. Recombinant forms are used to explore therapeutic strategies, including modulating its redox activity or interfering with its nuclear translocation. Additionally, AIFM1 mutations causing mitochondrial dysfunction are modeled using recombinant proteins to investigate disease pathogenesis and gene therapy approaches.
This protein’s duality as a metabolic regulator and apoptosis effector continues to drive interest in its structural biology and translational applications.
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