| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PSG7 |
| Uniprot No | Q13046 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 35-419aa |
| 氨基酸序列 | QVTIEAQPPKVSEGKDVLLLVHNLPQNLTGYIWYKGQIRDLYHYVTSYIVDGQIIKYGPAYSGRETVYSNASLLIQNVTQEDTGSYTLHIIKRGDGTGGVTGRFTFTLYLETPKPSISSSNFNPREATEAVILTCDPETPDASYLWWMNGQSLPMTHSLQLSETNRTLYLFGVTNYTAGPYECEIRNPVSASRSDPVTLNLLPKLPKPYITINNLNPRENKDVSTFTCEPKSENYTYIWWLNGQSLPVSPRVKRRIENRILILPSVTRNETGPYQCEIRDRYGGIRSDPVTLNVLYGPDLPRIYPSFTYYHSGQNLYLSCFADSNPPAQYSWTINGKFQLSGQKLSIPQITTKHSGLYACSVRNSATGKESSKSVTVRVSDWTLP |
| 预测分子量 | 48.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PSG7重组蛋白的参考文献示例(注：以下文献信息为模拟内容，实际研究中请核实具体文献)：
1. **文献名称**：*"Functional Characterization of Recombinant PSG7 in Immune Modulation"*
**作者**：Smith A, et al.
**摘要**：研究通过大肠杆菌表达系统成功制备了重组PSG7蛋白，并发现其可通过调节TGF-β通路抑制母体免疫反应，提示其在妊娠免疫耐受中的潜在作用。
2. **文献名称**：*"Expression and Purification of Human PSG7 in Mammalian Cell Systems"*
**作者**：Chen L, et al.
**摘要**：报道了利用HEK293细胞表达重组PSG7蛋白的优化方法，验证了其糖基化修饰对蛋白稳定性的影响，为后续功能研究提供高纯度样本。
3. **文献名称**：*"PSG7 as a Potential Biomarker in Preeclampsia: Insights from Recombinant Protein Studies"*
**作者**：Wang Y, et al.
**摘要**：通过检测孕妇血清及重组PSG7蛋白的功能实验，发现PSG7水平与子痫前期风险相关，可能通过调控血管生成因子减轻胎盘缺氧损伤。
4. **文献名称**：*"Structural Analysis of Recombinant PSG7 Reveals Unique Domain Interactions"*
**作者**：Gomez R, et al.
**摘要**：利用X射线晶体学解析了重组PSG7蛋白的三维结构，发现其N端IgV结构域与CEACAM家族其他成员存在差异，可能解释其特异性配体结合能力。
**提示**：实际研究中建议通过PubMed、Web of Science等平台以关键词“PSG7 recombinant protein”或“pregnancy-specific glycoprotein 7”检索最新文献，或结合PSG家族(如PSG1. PSG11)相关研究扩展信息。
PSG7 (Pregnancy-Specific Glycoprotein 7) is a member of the pregnancy-specific glycoprotein family, which belongs to the carcinoembryonic antigen (CEA) cell adhesion molecule (CEACAM) superfamily. These proteins are predominantly expressed in the placenta during pregnancy and are thought to play critical roles in maternal-fetal immunomodulation, trophoblast function, and placental development. PSG7. encoded by the human PSG7 gene on chromosome 19q13.2. shares structural homology with other PSG family members, featuring an N-terminal immunoglobulin variable-like (IgV) domain followed by multiple immunoglobulin constant-like (IgC) domains.
As a recombinant protein, PSG7 is typically produced using expression systems like mammalian cells (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications, particularly glycosylation, which is essential for its biological activity. Recombinant PSG7 enables researchers to study its interactions with immune cells, such as monocytes, dendritic cells, and regulatory T cells (Tregs), which are implicated in establishing immune tolerance at the maternal-fetal interface. Studies suggest PSG7 may modulate cytokine secretion (e.g., TGF-β, IL-10) and inhibit pro-inflammatory responses, thereby supporting fetal survival.
Beyond pregnancy, PSG7 has been investigated in pathological contexts, including cancer, where aberrant expression may influence tumor progression via immune evasion or angiogenesis. Recombinant PSG7 serves as a tool for developing diagnostic assays (e.g., monitoring pregnancy complications like preeclampsia) and exploring therapeutic strategies targeting immune regulation. Its role in CEACAM-mediated signaling pathways also makes it valuable for deciphering molecular mechanisms underlying placental disorders and cancer biology.
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