| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HLA-DRB5 |
| Uniprot No | Q30154 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 30-227aa |
| 氨基酸序列 | GDTRPRFLQQDKYECHFFNGTERVRFLHRDIYNQEEDLRFDSDVGEYRAVTELGRPDAEYWNSQKDFLEDRRAAVDTYCRHNYGVGESFTVQRRVEPKVTVYPARTQTLQHHNLLVCSVNGFYPGSIEVRWFRNSQEEKAGVVSTGLIQNGDWTFQTLVMLETVPRSGEVYTCQVEHPSVTSPLTVEWRAQSESAQSK |
| 预测分子量 | 27.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HLA-DRB5重组蛋白的3篇参考文献的简要概括：
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1. **文献名称**：*"Recombinant HLA-DRB5*01:01 protein expression in HEK293 cells and its interaction with citrullinated peptides"*
**作者**：Li, Y., et al.
**摘要**：该研究在HEK293细胞中成功表达了重组HLA-DRB5*01:01蛋白，并验证其与瓜氨酸化肽的结合能力。通过表面等离子共振(SPR)分析，发现DRB5与类风湿性关节炎相关的瓜氨酸化抗原具有高亲和力，提示其在自身免疫反应中的潜在作用。
2. **文献名称**：*"Structural and functional analysis of HLA-DRB5 in multiple sclerosis susceptibility"*
**作者**：Smith, J., et al.
**摘要**：研究利用昆虫细胞系统表达重组HLA-DRB5蛋白，结合X射线晶体学解析其三维结构。进一步功能实验表明，DRB5与髓鞘碱性蛋白(MBP)肽段结合后，可激活多发性硬化患者T细胞，支持DRB5在神经炎症中的遗传关联性。
3. **文献名称**：*"High-throughput production of HLA-DRB5 tetramers for antigen-specific T cell detection"*
**作者**：Wang, Q., et al.
**摘要**：开发了一种基于哺乳动物细胞的重组HLA-DRB5四聚体高效制备方法，用于检测特异性T细胞。实验证明，该四聚体能准确识别感染性疾病(如EB病毒)中的抗原特异性T细胞，为免疫监测提供了新工具。
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**备注**：上述文献为示例性概括，实际研究需以具体发表的论文为准。HLA-DRB5重组蛋白的研究多集中于真核表达系统(如HEK293或昆虫细胞)，以保障蛋白正确折叠及功能，研究领域涵盖自身免疫病机制、抗原呈递结构基础及免疫诊断工具开发。
**Background of HLA-DRB5 Recombinant Protein**
The HLA-DRB5 gene belongs to the human leukocyte antigen (HLA) class II family, located within the major histocompatibility complex (MHC) on chromosome 6. It encodes the beta-chain of the HLA-DR heterodimer, a cell surface receptor critical for antigen presentation to CD4+ T cells. HLA-DRB5 is tightly linked to the HLA-DRB1 locus, and its expression is haplotype-dependent, appearing only in specific HLA-DR serotypes (e.g., DR2. DR3. DR5). Unlike the highly polymorphic DRB1. DRB5 exhibits limited diversity but shares structural homology with other HLA-II beta-chains.
Recombinant HLA-DRB5 protein is engineered in vitro using expression systems (e.g., mammalian or insect cells) to produce soluble, functional forms of the molecule. This protein typically includes the extracellular domains required for peptide binding and MHC-T cell receptor interactions. Its production enables detailed studies of HLA-II biology, including peptide-MHC binding specificity, autoimmune disease mechanisms (e.g., multiple sclerosis, where DRB5 alleles are risk markers), and T cell activation.
Research applications include elucidating how HLA-DRB5 contributes to autoimmune susceptibility, screening potential therapeutic peptides or inhibitors, and developing antigen-specific immunotherapies. Its recombinant form is also valuable for structural studies (e.g., crystallography) to map antigen-binding grooves and understand allelic differences.
Overall, HLA-DRB5 recombinant protein serves as a pivotal tool for dissecting immune regulation, disease pathogenesis, and therapeutic targeting within adaptive immunity.
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