| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DEFB128 |
| Uniprot No | Q7Z7B8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 19-93aa |
| 氨基酸序列 | ARLKKCFNKVTGYCRKKCKVGERYEIGCLSGKLCCANDEEEKKHVSFKKPHQHSGEKLSVLQDYIILPTITIFTV |
| 预测分子量 | 16.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是假设的DEFB128重组蛋白相关参考文献示例(实际文献可能需要根据最新研究补充)：
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1. **文献名称**：*Antimicrobial Activity of Recombinant Human Beta-Defensin 128 in the Male Reproductive System*
**作者**：Smith J, et al.
**摘要**：研究成功在大肠杆菌中表达重组DEFB128.证实其在男性生殖道中具有广谱抗菌活性，尤其对革兰氏阴性菌抑制作用显著。
2. **文献名称**：*Cloning and Functional Characterization of DEFB128 as a Novel Immunomodulatory Peptide*
**作者**：Lee H, et al.
**摘要**：通过原核表达系统纯化DEFB128重组蛋白，发现其可调节巨噬细胞炎症因子分泌，提示潜在免疫调节功能。
3. **文献名称**：*Expression Profiling and Antibacterial Mechanisms of DEFB128 in Mammalian Cells*
**作者**：Wang Y, et al.
**摘要**：利用哺乳动物细胞表达重组DEFB128.揭示其通过破坏细菌膜结构发挥抗菌作用，并验证其在生殖道上皮细胞中的天然表达。
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**注意**：DEFB128研究相对较少，以上为基于防御素家族共性假设的示例。建议通过PubMed或Web of Science以“DEFB128 recombinant”或“human beta-defensin 128”为关键词检索最新文献。
DEFB128. a member of the β-defensin family, is a small cationic host-defense peptide implicated in innate immunity and antimicrobial defense. These evolutionarily conserved peptides are characterized by a cysteine-stabilized β-sheet structure stabilized by six disulfide-bonded cysteines. While most β-defensins (e.g., hBD-1 to hBD-4) are broadly studied for their microbicidal and immunomodulatory roles, DEFB128 remains relatively undercharacterized. It is encoded by the DEFB128 gene, located within a defensin cluster on human chromosome 20. and is primarily expressed in reproductive tissues, including the epididymis and testes, suggesting potential roles in sperm maturation or urogenital tract protection.
Recombinant DEFB128 protein is engineered using heterologous expression systems (e.g., E. coli or yeast) to produce functional peptide for mechanistic studies. Like other β-defensins, it likely disrupts microbial membranes via electrostatic interactions between its positively charged regions and negatively charged pathogen surfaces, leading to cell lysis. Additionally, it may recruit immune cells by chemoattracting dendritic cells or T-lymphocytes via CCR6 receptor binding, bridging innate and adaptive immunity.
Research on DEFB128 is emerging, with studies exploring its antimicrobial spectrum, tissue-specific expression patterns, and potential therapeutic applications. Its unique expression in reproductive tissues has sparked interest in contraceptive development or treatments for genital infections. However, challenges persist in understanding its precise physiological roles, receptor interactions, and stability in vivo. Recombinant DEFB128 serves as a critical tool to dissect these functions, offering avenues for novel antimicrobial agents or immunotherapies in an era of escalating antibiotic resistance.
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