| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | U69 |
| Uniprot No | P24446 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 170-355aa |
| 氨基酸序列 | SEERSYSVVYVPHNKELCGQFCQPEKTMARVLGVGAYGKVFDLDKVAIKTANEDESVISAFIAGVIRAKSGADLLSHECVINNLLISNSVCMSHKVSLSRTYDIDLHKFEDWDVRNVMNYYSVFCKLADAVRFLNLKCRINHFDISPMNIFLNHKKEIIFDAVLADYSLSEMHPNYNGTCAIAKEY |
| 预测分子量 | 25.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于U69重组蛋白的3篇假设性参考文献示例(仅供参考，非真实文献)：
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**1. 文献名称**
"Functional characterization of HHV-6 U69 protein as a phosphotransferase involved in antiviral drug resistance"
**作者**：Yamamoto T, et al.
**摘要**：本研究揭示了人类疱疹病毒6型(HHV-6)U69蛋白通过磷酸化修饰抗病毒药物更昔洛韦(ganciclovir)，导致病毒耐药性的分子机制，并证明重组U69蛋白在体外具有显著酶活性。
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**2. 文献名称**
"Expression and purification of recombinant U69 protein from HHV-6B: Implications for structural analysis"
**作者**：Lei Z, et al.
**摘要**：报道了通过大肠杆菌系统高效表达HHV-6B U69重组蛋白的优化策略，利用亲和层析技术纯化获得高纯度蛋白，并验证其与核苷类似物的结合能力。
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**3. 文献名称**
"Structural insights into the catalytic domain of U69 kinase from HHV-6 by X-ray crystallography"
**作者**：Smith J, et al.
**摘要**：通过X射线晶体学解析了U69蛋白催化结构域的3D结构，揭示了其与宿主细胞激酶的差异，为设计靶向U69的抗病毒药物提供了结构基础。
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**提示**：以上文献为假设性示例，实际研究中建议通过PubMed或Google Scholar以关键词“U69 protein HHV-6”或“U69 kinase antiviral”检索真实文献。
**Background of U69 Recombinant Protein**
The U69 protein is a gene product encoded by human herpesvirus 6 (HHV-6) and HHV-7. members of the betaherpesvirus subfamily. These viruses are associated with various clinical conditions, including roseola infantum, neurological disorders, and complications in immunocompromised individuals. The U69 gene, conserved across HHV-6 and HHV-7. encodes a phosphotransferase enzyme implicated in viral replication and pathogenesis.
Functionally, U69 exhibits homology to protein kinases and is involved in phosphorylating viral and host substrates. This activity facilitates viral evasion of host immune responses, modulation of cellular signaling pathways, and promotion of viral persistence. Studies suggest that U69 plays a role in viral DNA replication and latency establishment, making it a potential target for antiviral therapies.
Recombinant U69 protein is typically produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable biochemical and functional studies. Its recombinant form allows researchers to analyze kinase activity, substrate specificity, and interactions with host proteins. Additionally, U69 recombinant protein serves as an antigen for antibody development and diagnostic assays, aiding in the detection of HHV-6/HHV-7 infections.
Research on U69 has expanded due to its clinical relevance, particularly in understanding herpesvirus-induced pathologies and developing targeted inhibitors. However, challenges remain in fully elucidating its mechanism of action and therapeutic potential. Current efforts focus on structural characterization and inhibitor screening to combat HHV-6/HHV-7-associated diseases.
In summary, U69 recombinant protein is a critical tool for dissecting viral mechanisms and advancing antiviral strategies, bridging molecular virology with translational medicine.
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