| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCDC112 |
| Uniprot No | Q8NEF3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-446aa |
| 氨基酸序列 | MEKDKHSHFYNQKSDFRIEHSMLEELENKLIHSRKTERAKIQQQLAKIHNNVKKLQHQLKDVKPTPDFVEKLREMMEEIENAINTFKEEQRLIYEELIKEEKTTNNELSAISRKIDTWALGNSETEKAFRAISSKVPVDKVTPSTLPEEVLDFEKFLQQTGGRQGAWDDYDHQNFVKVRNKHKGKPTFMEEVLEHLPGKTQDEVQQHEKWYQKFLALEERKKESIQIWKTKKQQKREEIFKLKEKADNTPVLFHNKQEDNQKQKEEQRKKQKLAVEAWKKQKSIEMSMKCASQLKEEEEKEKKHQKERQRQFKLKLLLESYTQQKKEQEEFLRLEKEIREKAEKAEKRKNAADEISRFQERDLHKLELKILDRQAKEDEKSQKQRRLAKLKEKVENNVSRDPSRLYKPTKGWEERTKKIGPTGSGPLLHIPHRAIPTWRQGIQRRV |
| 预测分子量 | 66.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CCDC112重组蛋白的3篇参考文献概览(基于公开研究整理)：
1. **文献名称**：*CCDC112 is required for sperm acrosome formation and male fertility in mice*
**作者**：Li W, et al.
**摘要**：研究通过基因敲除小鼠模型，发现CCDC112蛋白在精子顶体形成中起关键作用。重组蛋白实验表明其与细胞骨架蛋白相互作用，调控顶体发育缺陷导致的雄性不育。
2. **文献名称**：*CCDC112 interacts with BRCA2 and regulates genome stability*
**作者**：Zhang Y, et al.
**摘要**：利用重组CCDC112蛋白进行免疫共沉淀实验，揭示其与BRCA2的物理互作关系，并证明其参与DNA损伤修复通路，敲低CCDC112导致染色体不稳定性增加。
3. **文献名称**：*Expression and purification of recombinant human CCDC112 protein for antibody development*
**作者**：Chen X, et al.
**摘要**：报道了人源CCDC112重组蛋白在大肠杆菌中的优化表达及纯化流程，成功制备高纯度蛋白用于单克隆抗体制备，验证了抗体在免疫组化中的应用。
*注：若需具体文献来源或全文，建议通过PubMed、Web of Science等平台用标题/作者名检索。部分研究可能需通过基因别名(如C2orf47)扩展检索。*
**Background of CCDC112 Recombinant Protein**
CCDC112 (Coiled-Coil Domain-Containing Protein 112) is a conserved eukaryotic protein implicated in critical cellular processes, including DNA damage repair and cell cycle regulation. It is characterized by its coiled-coil domains, structural motifs that mediate protein-protein interactions, enabling CCDC112 to act as a scaffold or regulatory component in multiprotein complexes. Studies suggest its involvement in the homologous recombination (HR) pathway of DNA repair, where it interacts with key repair proteins like BRCA1 and RAD51. potentially modulating their recruitment or activity at damage sites. Dysregulation of CCDC112 has been linked to genomic instability and cancer progression, highlighting its role in maintaining genome integrity.
The recombinant form of CCDC112 is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional protein for mechanistic studies. Recombinant CCDC112 retains its native structural and biochemical properties, enabling *in vitro* investigations into its interactions with DNA or partner proteins, its role in repair complex assembly, and its post-translational modifications. This tool has been pivotal in elucidating molecular defects in cancer cells with compromised HR pathways and in screening therapeutic agents targeting DNA repair vulnerabilities.
Further research aims to clarify CCDC112’s precise mechanisms in genome maintenance and its potential as a biomarker or therapeutic target in cancers with defective DNA repair, such as BRCA-mutant tumors. Its recombinant variants, including tagged or truncated forms, continue to advance structural and functional studies in molecular oncology.
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