| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KAT14 |
| Uniprot No | Q9H8E8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-782aa |
| 氨基酸序列 | MDSSIHLSSLISRHDDEATRTSTSEGLEEGEVEGETLLIVESEDQASVDLSHDQSGDSLNSDEGDVSWMEEQLSYFCDKCQKWIPASQLREQLSYLKGDNFFRFTCSDCSADGKEQYERLKLTWQQVVMLAMYNLSLEGSGRQGYFRWKEDICAFIEKHWTFLLGNRKKTSTWWSTVAGCLSVGSPMYFRSGAQEFGEPGWWKLVHNKPPTMKPEGEKLSASTLKIKAASKPTLDPIITVEGLRKRASRNPVESAMELKEKRSRTQEAKDIRRAQKEAAGFLDRSTSSTPVKFISRGRRPDVILEKGEVIDFSSLSSSDRTPLTSPSPSPSLDFSAPGTPASHSATPSLLSEADLIPDVMPPQALFHDDDEMEGDGVIDPGMEYVPPPAGSVASGPVVGVRKKVRGPEQIKQEVESEEEKPDRMDIDSEDTDSNTSLQTRAREKRKPQLEKDTKPKEPRYTPVSIYEEKLLLKRLEACPGAVAMTPEARRLKRKLIVRQAKRDRGLPLFDLDQVVNAALLLVDGIYGAKEGGISRLPAGQATYRTTCQDFRILDRYQTSLPSRKGFRHQTTKFLYRLVGSEDMAVDQSIVSPYTSRILKPYIRRDYETKPPKLQLLSQIRSHLHRSDPHWTPEPDAPLDYCYVRPNHIPTINSMCQEFFWPGIDLSECLQYPDFSVVVLYKKVIIAFGFMVPDVKYNEAYISFLFVHPEWRRAGIATFMIYHLIQTCMGKDVTLHVSASNPAMLLYQKFGFKTEEYVLDFYDKYYPLESTECKHAFFLRLRR |
| 预测分子量 | 94.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KAT14重组蛋白的模拟参考文献示例(供参考，实际文献需通过学术数据库检索)：
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1. **标题**: *KAT14 interacts with the HBO1 complex to regulate histone acetylation and DNA replication*
**作者**: Smith J, et al.
**期刊**: *Mol Cell Biol* (2020)
**摘要**: 研究揭示了KAT14作为HBO1组蛋白乙酰转移酶复合物的调控亚基，通过重组蛋白实验证明其参与组蛋白H3K14乙酰化，并调控DNA复制起始阶段的染色质重塑。
2. **标题**: *Structural insights into KAT14 recombinant protein function in chromatin remodeling*
**作者**: Lee S, et al.
**期刊**: *Nat Struct Mol Biol* (2018)
**摘要**: 通过X射线晶体学解析KAT14重组蛋白的结构，发现其C端结构域与HBO1的结合界面，为理解其在染色质修饰复合物中的催化机制提供依据。
3. **标题**: *KAT14 knockdown impairs transcriptional elongation via altered histone acetylation patterns*
**作者**: Chen X, et al.
**期刊**: *Nucleic Acids Res* (2019)
**摘要**: 利用重组KAT14蛋白进行体外乙酰化实验，证明其通过调控RNA聚合酶II相关基因的组蛋白乙酰化水平，影响转录延伸过程。
4. **标题**: *Functional characterization of recombinant KAT14 in stem cell differentiation*
**作者**: Wang Y, et al.
**期刊**: *Cell Rep* (2021)
**摘要**: 研究通过体外重组蛋白实验和细胞模型，揭示KAT14通过乙酰化特定靶点调控多能干细胞向中胚层分化的分子通路。
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**注意**：以上文献为示例，实际研究需通过PubMed、Google Scholar等平台以“KAT14”、“Lysine Acetyltransferase 14”、“重组蛋白”等关键词检索。KAT14常与HBO1复合物、染色质修饰、转录调控相关。
KAT14 (lysine acetyltransferase 14), also known as CSRP2BP, is a member of the MYST family of histone acetyltransferases (HATs) that play critical roles in epigenetic regulation. It catalyzes the transfer of acetyl groups to lysine residues on histone proteins, modifying chromatin structure and influencing gene expression. KAT14 is distinct within the MYST family due to its unique domain organization, which includes a chromodomain and a conserved HAT domain. This protein is primarily associated with the ATAC (Ada-Two-A-containing) complex, a multisubunit coactivator involved in transcription initiation and chromatin remodeling.
Functionally, KAT14 contributes to diverse cellular processes, including DNA repair, cell cycle regulation, and differentiation. Studies suggest it interacts with transcription factors and coactivators to mediate specific gene programs, particularly in development and stress responses. Its role in acetylating histone H4 (at lysine 5 and 8) and non-histone substrates underscores its broad regulatory influence. Dysregulation of KAT14 has been implicated in diseases such as cancers, where aberrant acetylation patterns may drive oncogenic pathways.
Recombinant KAT14 protein is engineered for in vitro studies to dissect its enzymatic activity, substrate specificity, and molecular interactions. Produced via heterologous expression systems (e.g., E. coli or mammalian cells), it often includes affinity tags (e.g., His-tag) for purification. Researchers utilize this tool to explore HAT inhibitors, map protein-DNA interactions, or model epigenetic mechanisms. Its application extends to drug discovery, aiming to target acetylation-related disorders. Despite progress, KAT14's full regulatory network and physiological context remain active areas of investigation, reflecting its complexity in cellular signaling and disease biology.
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