| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CLCN3 |
| Uniprot No | P51790 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-818aa |
| 氨基酸序列 | MASMTGGQQMGRDPMESEQLFHRGYYRNSYNSITSASSDEELLDGAGVIM DFQTSEDDNLLDGDTAVGTHYTMTNGGSINSSTHLLDLLDEPIPGVGTYD DFHTIDWVREKCKDRERHRRINSKKKESAWEMTKSLYDAWSGWLVVTLTG LASGALAGLIDIAADWMTDLKEGICLSALWYNHEQCCWGSNETTFEERDK CPQWKTWAELIIGQAEGPGSYIMNYIMYIFWALSFAFLAVSLVKVFAPYA CGSGIPEIKTILSGFIIRGYLGKWTLMIKTITLVLAVASGLSLGKEGPLV HVACCCGNIFSYLFPKYSTNEAKKREVLSAASAAGVSVAFGAPIGGVLFS LEEVSYYFPLKTLWRSFFAALVAAFVLRSINPFGNSRLVLFYVEYHTPWY LFELFPFILLGVFGGLWGAFFIRANIAWCRRRKSTKFGKYPVLEVIIVAA ITAVIAFPNPYTRLNTSELIKELFTDCGPLESSSLCDYRNDMNASKIVDD IPDRPAGIGVYSAIWQLCLALIFKIIMTVFTFGIKVPSGLFIPSMAIGAI AGRIVGIAVEQLAYYHHDWFIFKEWCEVGADCITPGLYAMVGAAACLGGV TRMTVSLVVIVFELTGGLEYIVPLMAAVMTSKWVGDAFGREGIYEAHIRL NGYPFLDAKEEFTHTTLAADVMRPRRNDPPLAVLTQDNMTVDDIENMINE TSYNGFPVIMSKESQRLVGFALRRDLTIAIESARKKQEGIVGSSRVCFAQ HTPSLPAESPRPLKLRSILDMSPFTVTDHTPMEIVVDIFRKLGLRQCLVT HNGRLLGIITKKDILRHMAQTANQDPASIMFN |
| 预测分子量 | 93 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于CLCN3重组蛋白研究的参考文献(信息基于公开文献概括，非真实存在，仅作示例参考)：
1. **《Functional characterization of recombinant human CLCN3 chloride channel in Xenopus oocytes》**
- 作者：Smith A et al.
- 摘要：通过爪蟾卵母细胞表达重组人源CLCN3蛋白，证实其具有电压依赖性氯离子通道活性，并发现其参与细胞体积调节。
2. **《Structural insights into CLCN3 regulation by pH: Cryo-EM analysis of the recombinant protein》**
- 作者：Li X et al.
- 摘要：利用冷冻电镜解析CLCN3重组蛋白的pH敏感结构域，揭示其胞内羧基末端在酸激活通道功能中的关键作用。
3. **《CLCN3 knockout and recombinant rescue models reveal its role in neuronal excitability》**
- 作者：Chen Y et al.
- 摘要：通过构建CLCN3基因敲除小鼠及重组蛋白回补实验，证明CLCN3通过调节溶酶体氯离子浓度影响神经元兴奋性和癫痫易感性。
注：以上文献名为虚拟示例，实际研究需通过PubMed或Web of Science等平台检索真实文献。
**Background of CLCN3 Recombinant Protein**
CLCN3. a member of the CLC family of chloride channels/transporters, is a voltage-gated ion channel or transporter implicated in regulating chloride ion (Cl⁻) flux across cellular membranes. Encoded by the *CLCN3* gene, this protein is ubiquitously expressed, with notable presence in the brain, kidneys, and endocrine tissues. Structurally, CLCN3 features a characteristic double-barreled pore architecture formed by dimerization, enabling selective Cl⁻ conduction or H⁺/Cl⁻ exchange, depending on its functional mode.
CLCN3 plays diverse physiological roles, including cell volume regulation, lysosomal pH homeostasis, and synaptic vesicle acidification. Dysregulation of CLCN3 has been linked to neurological disorders (e.g., epilepsy, neurodegeneration), cancer progression, and renal dysfunction. Its involvement in intracellular organelle acidification also highlights its importance in cellular trafficking and autophagy.
Recombinant CLCN3 protein is engineered using heterologous expression systems (e.g., *E. coli*, mammalian cells) to study its biophysical properties, structure-function relationships, and interaction partners. Purification techniques, such as affinity chromatography, yield high-purity protein for *in vitro* assays, electrophysiological studies (e.g., patch-clamp), or antibody production. Researchers utilize CLCN3 recombinant protein to investigate its role in disease mechanisms, screen potential modulators, or validate genetic models.
Recent studies focus on its dual role as a channel or transporter, post-translational modifications (e.g., phosphorylation), and crosstalk with signaling pathways like mTOR or Wnt. These insights may inform therapeutic strategies targeting CLCN3-related pathologies. Overall, CLCN3 recombinant protein serves as a critical tool for unraveling the molecular basis of its function and dysfunction in health and disease.
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