| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ALPK1 |
| Uniprot No | Q96QP1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1017-1237aa |
| 氨基酸序列 | KYSKKSELWTAQETIVYLGDYLTVKKKGRQRNAFWVHHLHQEEILGRYVGKDYKEQKGLWHHFTDVERQMTAQHYVTEFNKRLYEQNIPTQIFYIPSTILLILEDKTIKGCISVEPYILGEFVKLSNNTKVVKTEYKATEYGLAYGHFSYEFSNHRDVVVDLQGWVTGNGKGLIYLTDPQIHSVDQKVFTTNFGKRGIFYFFNNQHVECNEICHRLSLTRP |
| 预测分子量 | 29.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ALPK1重组蛋白研究的示例参考文献(注：以下内容为示例性概括，实际文献需通过学术数据库查询)：
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1. **"Expression and Functional Characterization of Recombinant ALPK1 Kinase in Inflammatory Signaling"**
*作者：Wang X et al.*
**摘要**：本研究成功在大肠杆菌中表达并纯化了重组ALPK1蛋白，证实其具有ATP依赖的激酶活性。通过体外实验发现，ALPK1重组蛋白可磷酸化下游信号分子TIFA，激活NF-κB通路，提示其在细菌感染引发的先天免疫中的关键作用。
2. **"Structural Insights into ALPK1 Kinase Domain via Recombinant Protein Crystallography"**
*作者：Zhang Y et al.*
**摘要**：利用昆虫细胞系统表达重组ALPK1激酶结构域，通过X射线晶体学解析了其三维结构。研究揭示了ALPK1的ATP结合口袋特征，为针对该激酶的抑制剂设计提供了结构基础。
3. **"ALPK1 Recombinant Protein Variants Link to Metabolic Dysregulation in Type 2 Diabetes"**
*作者：Chen L et al.*
**摘要**：构建了ALPK1常见疾病相关突变体(如p.R1114C)的重组蛋白，发现突变体导致激酶活性显著降低，并与胰岛素信号通路异常相关，提示ALPK1在代谢性疾病中的潜在机制。
4. **"Development of a High-Throughput Screening Assay Using Recombinant ALPK1 for Drug Discovery"**
*作者：Kim S et al.*
**摘要**：优化了ALPK1重组蛋白在哺乳动物细胞中的表达体系，并建立基于荧光共振能量转移(FRET)的激酶活性检测方法，用于筛选小分子抑制剂，为炎症性疾病治疗提供新策略。
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建议通过PubMed、Web of Science等平台以“ALPK1 recombinant protein”“ALPK1 kinase activity”等关键词检索最新文献以获取真实数据。
ALPK1 (Alpha-protein kinase 1) is a serine/threonine kinase involved in regulating innate immune responses and inflammatory pathways. It is encoded by the ALPK1 gene, which is conserved across vertebrates and consists of an N-terminal kinase domain and a C-terminal regulatory domain. ALPK1 plays a critical role in sensing bacterial metabolites, such as ADP-heptose—a pathogen-associated molecular pattern (PAMP) derived from Gram-negative bacteria. Upon activation, ALPK1 phosphorylates TRAF-interacting protein (TRAIP), triggering downstream NF-κB and MAPK signaling cascades, leading to pro-inflammatory cytokine production (e.g., IL-6. IL-8) and immune cell recruitment.
Dysregulation of ALPK1 has been linked to several human diseases. Genetic variants of ALPK1 are associated with chronic kidney disease, gout, and cardiovascular disorders, particularly in East Asian populations. For example, specific ALPK1 mutations correlate with enhanced kinase activity, promoting hyperuricemia and gout pathogenesis. Additionally, ALPK1 overexpression is implicated in cardiac remodeling and diabetic cardiomyopathy, highlighting its role in metabolic and stress-related pathologies.
Recombinant ALPK1 protein, typically produced in Escherichia coli or mammalian expression systems, is a key tool for studying its biochemical properties, substrate interactions, and signaling mechanisms. Purified ALPK1 retains kinase activity and is used in in vitro phosphorylation assays, structural studies (e.g., crystallography), and high-throughput drug screening. Tagged versions (e.g., His-, GST-tagged) facilitate protein purification and interaction analyses. Research on recombinant ALPK1 aids in elucidating its role in immunity and disease, offering potential therapeutic targets for inflammatory and metabolic disorders.
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