| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Y471H |
| Uniprot No | P00750 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 36-562aa |
| 氨基酸序列 | SYQVICRDEKTQMIYQQHQSWLRPVLRSNRVEYCWCNSGRAQCHSVPVKSCSEPRCFNGGTCQQALYFSDFVCQCPEGFAGKCCEIDTRATCYEDQGISYRGTWSTAESGAECTNWNSSALAQKPYSGRRPDAIRLGLGNHNYCRNPDRDSKPWCYVFKAGKYSSEFCSTPACSEGNSDCYFGNGSAYRGTHSLTESGASCLPWNSMILIGKVYTAQNPSAQALGLGKHNYCRNPDGDAKPWCHVLKNRRLTWEYCDVPSCSTCGLRQYSQPQFRIKGGLFADIASHPWQAAIFAKHRRSPGERFLCGGILISSCWILSAAHCFQERFPPHHLTVILGRTYRVVPGEEEQKFEVEKYIVHKEFDDDTYDNDIALLQLKSDSSRCAQESSVVRTVCLPPADLQLPDWTECELSGYGKHEALSPFYSERLKEAHVRLHPSSRCTSQHLLNRTVTDNMLCAGDTRSGGPQANLHDACQGDSGGPLVCLNDGRMTLVGIISWGLGCGQKDVPGVYTKVTNYLDWIRDNMRP |
| 预测分子量 | 62.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Y471H重组蛋白的模拟参考文献示例(注：文献为虚构，仅供示例参考)：
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1. **文献名称**：*Structural and Functional Analysis of the Y471H Mutation in Recombinant EGFR Kinase Domain*
**作者**：Chen, L., et al.
**摘要**：本研究通过X射线晶体学解析了Y471H突变体EGFR激酶结构，发现该突变导致活性位点构象改变，显著降低其自磷酸化能力，并影响下游信号通路的激活。
2. **文献名称**：*Y471H Recombinant Protein Exhibits Altered Substrate Binding in vitro*
**作者**：Martinez, R., & Kim, S.
**摘要**：利用等温滴定量热法(ITC)和酶动力学实验，证明Y471H突变削弱了重组蛋白与底物的结合亲和力，导致催化效率下降约70%，提示该位点对功能至关重要。
3. **文献名称**：*Thermodynamic Stability of Y471H Mutant Recombinant Protein in Cancer Models*
**作者**：Watanabe, H., et al.
**摘要**：通过差示扫描量热法(DSC)和细胞实验，发现Y471H突变体蛋白的热稳定性降低，且在肿瘤细胞中过表达时显著抑制增殖，提示其潜在治疗应用价值。
4. **文献名称**：*Computational Modeling of Y471H-Induced Conformational Changes in Recombinant Tyrosine Kinases*
**作者**：Patel, A., et al.
**摘要**：结合分子动力学模拟和体外验证，揭示Y471H突变通过破坏局部氢键网络，诱导激酶结构域从活性构象向非活性状态转变，为靶向药物设计提供依据。
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**注意**：以上文献为模拟生成，实际研究中请通过PubMed、Google Scholar等平台检索真实文献(关键词如“Y471H recombinant protein”+具体蛋白名称/研究领域)。
**Background of Y471H Recombinant Protein**
The Y471H recombinant protein is a genetically engineered variant of a parent protein, typically designed to study the functional or structural impact of a specific amino acid substitution—tyrosine (Y) to histidine (H) at position 471. Such mutations are often introduced to investigate residue-specific roles in protein activity, stability, or interactions. For example, in viral proteins (e.g., SARS-CoV-2 spike protein), analogous mutations (e.g., Y453H in animal models) have been linked to altered receptor-binding affinity or immune evasion. The "Y471H" designation suggests a similar focus on understanding how this substitution modulates biological behavior.
Recombinant production involves cloning the mutated gene into expression systems (e.g., *E. coli*, yeast, or mammalian cells), followed by purification using affinity chromatography. The protein’s applications span structural studies (e.g., crystallography or cryo-EM), functional assays (e.g., binding kinetics or enzymatic activity), or vaccine/drug development. The Y-to-H change may influence hydrogen bonding, charge distribution, or conformational dynamics, potentially affecting interactions with ligands, antibodies, or cellular receptors.
Research on Y471H may also explore its role in disease mechanisms, such as pathogenicity or drug resistance. For instance, in oncology, analogous mutations in kinases or signaling proteins can alter signaling pathways. Characterization typically includes biophysical methods (e.g., circular dichroism, thermal shift assays) and functional validations (e.g., cell-based assays). Overall, Y471H recombinant proteins serve as critical tools for dissecting molecular mechanisms and guiding therapeutic innovations.
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