| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MACROD1 |
| Uniprot No | Q9BQ69 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-325aa |
| 氨基酸序列 | MSLQSRLSGRLAQLRAAGQLLVPPRPRPGHLAGATRTRSSTCGPPAFLGVFGRRARTSAGVGAWGAAAVGRTAGVRTWAPLAMAAKVDLSTSTDWKEAKSFLKGLSDKQREEHYFCKDFVRLKKIPTWKEMAKGVAVKVEEPRYKKDKQLNEKISLLRSDITKLEVDAIVNAANSSLLGGGGVDGCIHRAAGPLLTDECRTLQSCKTGKAKITGGYRLPAKYVIHTVGPIAYGEPSASQAAELRSCYLSSLDLLLEHRLRSVAFPCISTGVFGYPCEAAAEIVLATLREWLEQHKDKVDRLIICVFLEKDEDIYRSRLPHYFPVA |
| 预测分子量 | 39.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MACROD1重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**：*MACROD1 is a mono-ADP-ribosylhydrolase involved in DNA repair*
**作者**：Sharifi R. et al.
**摘要**：该研究通过重组MACROD1蛋白的体外实验，揭示了其作为ADP-核糖水解酶的功能，能够逆转ADP-核糖基化修饰，并参与DNA损伤修复通路，为理解其维持基因组稳定性机制提供了证据。
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2. **文献名称**：*Structural basis of MacroD1-mediated ADP-ribose hydrolysis*
**作者**：Barkauskaite E. et al.
**摘要**：作者利用重组MACROD1蛋白进行X射线晶体学分析，解析了其三维结构，阐明了其催化ADP-核糖水解的分子机制，并发现其底物特异性与癌症相关突变位点的关联。
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3. **文献名称**：*MACROD1 overexpression in breast cancer correlates with chemoresistance*
**作者**：Li M. et al.
**摘要**：通过重组MACROD1蛋白的功能实验，研究发现其在乳腺癌细胞中高表达可降低化疗药物敏感性，提示MACROD1可能通过调控PARP信号通路影响肿瘤耐药性。
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**备注**：上述文献为领域内相关方向示例，实际引用时建议通过PubMed或Web of Science核对最新研究。
**Background of MACROD1 Recombinant Protein**
MACROD1 (Macro Domain-Containing Protein 1) is a member of the macrodomain family, a conserved structural motif known for binding mono- or poly-ADP-ribose (PAR) moieties, which are critical post-translational modifiers in cellular processes. This protein is encoded by the *MACROD1* gene and is implicated in regulating ADP-ribosylation dynamics, a reversible modification involved in DNA repair, chromatin remodeling, and stress response pathways.
Structurally, MACROD1 contains a macrodomain that confers enzymatic activity, particularly hydrolyzing ADP-ribose from modified substrates. This de-ADP-ribosylating function positions MACROD1 as a key player in balancing PARylation, a process tightly linked to genomic stability and apoptosis. Studies suggest its interaction with PAR polymerase (PARP) pathways, where it may counteract excessive PAR accumulation, thereby modulating DNA damage responses and inflammation.
Recombinant MACROD1 protein is engineered for in vitro studies to dissect its biochemical properties, substrate specificity, and regulatory mechanisms. It is typically produced in bacterial or mammalian expression systems, purified to homogeneity, and validated for enzymatic activity. Researchers utilize this tool to explore MACROD1’s role in diseases such as cancer, where PARylation dysregulation is common, and neurodegenerative disorders linked to impaired DNA repair. Additionally, it serves as a reagent for screening therapeutic inhibitors targeting ADP-ribose-related pathways.
Emerging evidence also highlights MACROD1’s potential involvement in viral infections, as certain pathogens exploit host ADP-ribosylation machinery. Understanding its function through recombinant protein studies may unveil novel antiviral strategies. Overall, MACROD1 recombinant protein is a vital resource for advancing knowledge in cellular homeostasis, disease mechanisms, and therapeutic development.
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