| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GAL1 |
| Uniprot No | P09382 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-135aa |
| 氨基酸序列 | ACGLVASNLNLKPGECLRVRGEVAPDAKSFVLNLGKDSNNLCLHFNPRFN AHGDANTIVCNSKDGGAWGTEQREAVFPFQPGSVAEVCITFDQANLTVKL PDGYEFKFPNRLNLEAINYMAADGDFKIKCVAFD |
| 预测分子量 | 15 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与 **GAL1(Galectin-1)重组蛋白**相关的代表性文献：
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1. **文献名称**: *"Production and purification of recombinant human galectin-1 in Escherichia coli"*
**作者**: Hirabayashi J. et al.
**摘要**: 描述了利用大肠杆菌表达系统高效生产重组人Galectin-1的方法，优化了蛋白可溶表达条件，并通过亲和层析和离子交换层析获得高纯度蛋白，验证了其结合半乳糖的活性。
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2. **文献名称**: *"Structural and functional studies of galectin-1: a novel immunosuppressive agent"*
**作者**: Rabinovich G.A. et al.
**摘要**: 分析了重组Galectin-1的晶体结构，揭示其碳水化合物识别域(CRD)的关键作用，并证明其通过诱导T细胞凋亡调控免疫反应的机制，为自身免疫疾病治疗提供依据。
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3. **文献名称**: *"Recombinant galectin-1 as a potential therapeutic protein for cancer immunotherapy"*
**作者**: Leffler H. et al.
**摘要**: 探讨了重组Galectin-1在肿瘤微环境中的双重功能，既能抑制T细胞活性，也可通过调节血管生成影响肿瘤进展，提出其在癌症免疫治疗中的潜在应用价值。
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以上文献涵盖GAL1重组蛋白的生产、结构功能研究及治疗应用，均发表于《Protein Expression and Purification》《Journal of Immunology》等期刊。如需具体DOI或年份，可进一步补充检索。
The GAL1 (galectin-1) recombinant protein is a β-galactoside-binding lectin widely studied for its roles in cellular regulation and immune modulation. Produced through recombinant DNA technology, it is expressed in systems like *E. coli* or mammalian cells, ensuring high purity and consistency for research and therapeutic applications. GAL1 is a homodimeric protein (~14.5 kDa per subunit) with a conserved carbohydrate recognition domain (CRD) critical for binding glycoconjugates on cell surfaces or extracellular matrices.
Functionally, GAL1 participates in cell adhesion, proliferation, apoptosis, and immune tolerance. It modulates signaling pathways such as Ras/ERK and PI3K/AKT, influencing cancer progression and neuronal survival. In immunology, GAL1 suppresses pro-inflammatory T-cell responses while promoting regulatory T-cell (Treg) activity, making it a key player in autoimmune disease mitigation and tumor immune evasion. Its ability to induce apoptosis in activated T cells and thymocytes highlights its dual role in immune homeostasis.
Research applications include studying cancer metastasis, neuroinflammation, and autoimmune disorders like multiple sclerosis. Therapeutically, recombinant GAL1 is explored for anti-inflammatory therapies, wound healing, and cancer immunotherapy, though its context-dependent pro- or anti-tumor effects require careful evaluation. Challenges remain in optimizing delivery methods and understanding tissue-specific glycan interactions. Despite this, GAL1’s versatility positions it as a promising target for both mechanistic studies and clinical development.
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