| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | aprN |
| Uniprot No | P35835 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-381aa |
| 氨基酸序列 | MRSKKLWISLLFALTLIFTMAFSNMSAQAAGKSSTEKKYIVGFKQTMSAMSSAKKKDVISEKGGKVQKQFKYVNAAAATLDEKAVKELKKDPSVAYVEEDHIAHEYAQSVPYGISQIKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLNVRGGASFVPSETNPYQDGSSHGTHVAGTIAALNNSIGVLGVAPSASLYAVKVLDSTGSGQYSWIINGIEWAISNNMDVINMSLGGPTGSTALKTVVDKAVSSGIVVAAAAGNEGSSGSTSTVGYPAKYPSTIAVGAVNSSNQRASFSSVGSELDVMAPGVSIQSTLPGGTYGAYNGTSMATPHVAGAAALILSKHPTWTNAQVRDRLESTATYLGNSFYYGKGLINVQAAAQ |
| 预测分子量 | 39,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于aprN重组蛋白的模拟参考文献示例(仅供参考，建议通过学术数据库查询真实文献)：
1. **《Heterologous Expression and Characterization of aprN Protease from Bacillus subtilis》**
- 作者：Zhang L, Wang Y, et al.
- 摘要：本研究在大肠杆菌中成功表达了来源于枯草芽孢杆菌的aprN重组蛋白酶，并通过亲和层析纯化。酶学分析表明，该蛋白酶在pH 9.0和50°C时活性最高，且在洗涤剂中表现出良好的稳定性，提示其工业应用潜力。
2. **《Optimization of aprN Production in Pichia pastoris for Industrial Applications》**
- 作者：Chen H, Kim SJ, et al.
- 摘要：通过毕赤酵母表达系统高效分泌表达aprN重组蛋白，优化发酵条件后产量提升3倍。纯化蛋白在降解胶原蛋白和酪蛋白中显示高效催化活性，适用于食品加工或生物制药领域。
3. **《Structural Analysis of aprN Recombinant Protein Reveals Thermostability Determinants》**
- 作者：Müller R, Schmidt A.
- 摘要：利用X射线晶体学解析了aprN重组蛋白的三维结构，发现其N端结构域中的特定氨基酸残基对热稳定性起关键作用。通过定点突变进一步提高了该酶的耐高温性能。
4. **《Functional Screening of aprN Mutants for Enhanced Detergent Compatibility》**
- 作者：Tanaka K, et al.
- 摘要：通过定向进化获得多个aprN突变体，其中突变体D126G在含表面活性剂的溶液中活性保留率达85%，显著优于野生型，为开发高效生物洗涤剂提供了新候选酶。
注：以上文献为模拟内容，实际研究需查询权威数据库(如PubMed、Web of Science)。
**Background of aprN Recombinant Protein**
The aprN recombinant protein is derived from the aprN gene, which encodes a protease or related enzymatic protein in specific bacterial species, such as *Pseudomonas* or related genera. aprN-associated proteases are often linked to extracellular enzyme production, playing roles in nutrient acquisition, biofilm formation, or host-pathogen interactions. Recombinant aprN protein is generated through genetic engineering, where the aprN gene is cloned into expression vectors (e.g., *E. coli* or yeast systems) to enable large-scale production. This approach allows for high-purity protein yields, facilitating functional and structural studies.
Interest in aprN stems from its potential applications in biotechnology and medicine. For instance, proteases like those encoded by aprN are valuable in industrial processes, including detergent formulation, waste management, or food processing, due to their ability to degrade organic substrates. In pathogenic bacteria, aprN proteases may contribute to virulence, making them targets for antimicrobial drug development. Recombinant aprN protein enables researchers to study its enzymatic kinetics, substrate specificity, and inhibition mechanisms, aiding in the design of enzyme inhibitors or therapeutic agents.
Additionally, aprN recombinant protein serves as a tool in diagnostics, such as antibody production for detecting bacterial infections. Its study also contributes to understanding bacterial adaptation mechanisms, including antibiotic resistance or environmental stress responses. Overall, aprN recombinant protein bridges fundamental microbiology with applied research, offering insights into both microbial physiology and biotechnological innovation.
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