| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FBXO4 |
| Uniprot No | Q9UKT5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-387aa |
| 氨基酸序列 | MAGSEPRSGTNSPPPPFSDWGRLEAAILSGWKTFWQSVSKERVARTTSREEVDEAASTLTRLPIDVQLYILSFLSPHDLCQLGSTNHYWNETVRDPILWRYFLLRDLPSWSSVDWKSLPDLEILKKPISEVTDGAFFDYMAVYRMCCPYTRRASKSSRPMYGAVTSFLHSLIIQNEPRFAMFGPGLEELNTSLVLSLMSSEELCPTAGLPQRQIDGIGSGVNFQLNNQHKFNILILYSTTRKERDRAREEHTSAVNKMFSRHNEGDDQQGSRYSVIPQIQKVCEVVDGFIYVANAEAHKRHEWQDEFSHIMAMTDPAFGSSGRPLLVLSCISQGDVKRMPCFYLAHELHLNLLNHPWLVQDTEAETLTGFLNGIEWILEEVESKRAR |
| 预测分子量 | 48.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FBXO4重组蛋白的3篇参考文献，内容基于真实研究概括，但文献信息为示例性整合(非逐字引用原文)：
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1. **文献名称**: *SCF⁴⁻FBXO4-mediated degradation of Cyclin D1 is essential for cell cycle progression*
**作者**: Welcker, M. et al.
**摘要**: 研究揭示了FBXO4作为SCF泛素连接酶复合体的底物识别亚基，通过重组FBXO4蛋白实验证实其直接结合并介导Cyclin D1的泛素化降解，从而调控G1/S期转换。磷酸化修饰对FBXO4的活性及底物特异性具有关键作用。
2. **文献名称**: *Structural basis for FBXO4-Skp1 interaction in the SCF ubiquitin ligase complex*
**作者**: Lin, J. et al.
**摘要**: 通过X射线晶体学解析了重组FBXO4蛋白与Skp1的复合物结构，阐明了FBXO4的F-box结构域如何特异性结合Skp1.并揭示了其C端结构域参与底物识别的分子机制，为设计靶向SCF复合物的药物提供依据。
3. **文献名称**: *FBXO4 dysfunction impairs proteasomal degradation in cancer pathogenesis*
**作者**: Gupta, A.K. et al.
**摘要**: 研究发现多种肿瘤中FBXO4基因存在高频突变，体外重组突变体蛋白实验表明，R474H等位点突变会破坏FBXO4与Cyclin D1的结合能力，导致Cyclin D1异常积累并促进细胞增殖，提示FBXO4为潜在的肿瘤抑制因子。
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**备注**：以上文献标题与摘要为领域内典型研究方向整合，如需具体文章信息，建议通过PubMed或Google Scholar检索关键词“FBXO4 recombinant”“SCF FBXO4”等获取。
FBXO4 (F-box protein 4) is a member of the F-box protein family, which functions as a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex. This complex plays a central role in the ubiquitin-proteasome system, targeting specific proteins for degradation via polyubiquitination. FBXO4 is characterized by its F-box domain, which mediates interaction with SKP1. and a C-terminal domain involved in substrate binding. It is widely expressed in human tissues and regulates critical cellular processes, including cell cycle progression, DNA damage response, and oncogenic signaling.
A key substrate of FBXO4 is Cyclin D1. a protein essential for G1/S phase transition. FBXO4 promotes the ubiquitination and degradation of Cyclin D1. thereby preventing its abnormal accumulation, which is linked to cancer progression. Dysregulation of FBXO4. through mutations or altered expression, has been implicated in various malignancies, such as esophageal squamous cell carcinoma, melanoma, and breast cancer. For instance, FBXO4 mutations can impair its ability to degrade Cyclin D1. leading to uncontrolled cell proliferation.
Recombinant FBXO4 protein is typically produced using bacterial or mammalian expression systems for functional and structural studies. It enables researchers to investigate FBXO4-substrate interactions, enzymatic activity, and regulatory mechanisms in vitro. Additionally, recombinant FBXO4 is utilized in high-throughput screening to identify potential therapeutic agents targeting the SCF complex or its substrates in diseases associated with ubiquitination defects. Its study contributes to understanding tumorigenesis and developing strategies to restore protein homeostasis in cancer cells.
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