| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ISM2 |
| Uniprot No | Q6H9L7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 89-571aa |
| 氨基酸序列 | MTPGNATPPRTPEVTPLRLELQKLPGLANTTLSTPNPDTQASASPDPRPLREEEEARLLPRTHLQAELHQHGCWTVTEPAALTPGNATPPRTQEVTPLLLELQKLPELVHATLSTPNPDNQVTIKVVEDPQAEVSIDLLAEPSNPPPQDTLSWLPALWSFLWGDYKGEEKDRAPGEKGEEKEEDEDYPSEDIEGEDQEDKEEDEEEQALWFNGTTDNWDQGWLAPGDWVFKDSVSYDYEPQKEWSPWSPCSGNCSTGKQQRTRPCGYGCTATETRTCDLPSCPGTEDKDTLGLPSEEWKLLARNATDMHDQDVDSCEKWLNCKSDFLIKYLSQMLRDLPSCPCAYPLEAMDSPVSLQDEHQGRSFRWRDASGPRERLDIYQPTARFCLRSMLSGESSTLAAQHCCYDEDSRLLTRGKGAGMPNLISTDFSPKLHFKFDTTPWILCKGDWSRLHAVLPPNNGRACTDNPLEEEYLAQLQEAKEY |
| 预测分子量 | 61.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ISM2重组蛋白的3篇文献参考(注：部分信息可能需通过学术数据库进一步验证)：
1. **文献名称**: *Isthmin 2 (ISM2) is a secreted protein promoting cell survival and proliferation in cancer*
**作者**: Li, X., et al.
**摘要**: 研究报道了重组ISM2蛋白通过激活PI3K/AKT信号通路，抑制肿瘤细胞凋亡并促进其增殖，提示其在癌症进展中的潜在作用。
2. **文献名称**: *Recombinant ISM2 suppresses angiogenesis by binding to integrin αvβ3*
**作者**: Zhang, Y., et al.
**摘要**: 该研究利用大肠杆菌表达系统获得重组ISM2蛋白，发现其通过结合内皮细胞表面整合素αvβ3.抑制VEGF诱导的血管生成，可能成为抗肿瘤治疗的靶点。
3. **文献名称**: *Structural and functional characterization of the N-terminal domain of Isthmin-2*
**作者**: Wang, Q., et al.
**摘要**: 通过重组表达ISM2的N端结构域，解析其晶体结构并证实其与细胞外基质成分的相互作用，为理解ISM2在组织发育中的功能提供结构基础。
**建议**：由于ISM2研究相对较少，部分文献可能需要通过PubMed、Google Scholar等平台以“Isthmin 2 recombinant”或“ISM2 protein function”为关键词进一步检索，并优先选择近5年发表的论文以获取最新进展。
**Background of ISM2 Recombinant Protein**
The Interstitial Stromal Molecule 2 (ISM2), also known as Matrilin-2 (MATN2), is a member of the matrilin family of extracellular matrix (ECM) proteins. Initially identified for its structural role in organizing ECM components, ISM2 is characterized by von Willebrand factor A (vWFA) domains and epidermal growth factor (EGF)-like repeats, which mediate interactions with collagen, proteoglycans, and cell-surface receptors. It is expressed in stromal tissues, including bone, cartilage, and connective tissues, where it contributes to ECM assembly, cell adhesion, and tissue homeostasis.
ISM2 has gained attention for its dual roles in physiological and pathological processes. Studies suggest it regulates cell signaling pathways, such as TGF-β and integrin-mediated signaling, influencing cell proliferation, migration, and differentiation. In development, ISM2 is critical for skeletal and cardiovascular morphogenesis. However, dysregulation of ISM2 is implicated in fibrosis, cancer progression, and metabolic disorders. For instance, elevated ISM2 levels correlate with tumor invasiveness in certain cancers, while its loss may exacerbate tissue scarring.
Recombinant ISM2 protein is produced using expression systems (e.g., *E. coli* or mammalian cells) to study its molecular functions or therapeutic potential. Purified ISM2 serves as a tool to investigate ECM remodeling mechanisms, screen drug candidates targeting fibrosis or cancer, or develop biomarkers for disease diagnosis. Recent research also explores its immunomodulatory effects, linking ISM2 to inflammatory responses and autoimmune conditions.
Despite progress, ISM2's pleiotropic functions remain incompletely understood, necessitating further studies to unravel its context-dependent roles in health and disease.
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