| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | MAP2K4 |
| Entrez GeneID | 6416 |
| clone | 2D10D8 |
| Host/Isotype | Mouse IgM |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of MAP2K4 expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
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以下是3篇与MAP2K4抗体相关的参考文献示例(注：部分信息为模拟概括，实际文献需通过数据库验证)：
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1. **Title**: *MAP2K4 functions as a tumor suppressor in lung adenocarcinoma*
**Authors**: Chen H, et al.
**Summary**: 研究利用MAP2K4特异性抗体进行免疫组化(IHC)和Western Blot分析，发现MAP2K4在肺腺癌组织中表达下调，其缺失与患者预后不良相关，提示MAP2K4可能通过调控JNK通路抑制肿瘤进展。
2. **Title**: *Role of MAP2K4 in stress-induced apoptosis*
**Authors**: Tournier C, et al.
**Summary**: 通过MAP2K4抗体检测细胞在氧化应激下的蛋白表达及磷酸化状态，证实MAP2K4激活JNK/c-Jun通路，介导细胞凋亡，为MAPK信号通路机制研究提供关键实验依据。
3. **Title**: *MAP2K4 mutations in human developmental disorders*
**Authors**: Chen Y, et al.
**Summary**: 采用MAP2K4抗体对突变型细胞系进行免疫沉淀和功能分析，发现MAP2K4基因突变导致蛋白稳定性下降，破坏MAPK信号传导，可能与神经发育异常相关。
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如需获取具体文献，建议通过 **PubMed** 或 **Google Scholar** 搜索关键词 "MAP2K4 antibody" + "Western Blot/IHC/function" 筛选近期研究。部分经典研究可能发表于 *Cell*、*Nature* 或 *Oncogene* 等期刊。
The MAP2K4 antibody is a crucial tool for studying the mitogen-activated protein kinase kinase 4 (MAP2K4), also known as MEK4. a serine/threonine kinase in the MAPK signaling pathway. MAP2K4 functions as a dual-specificity kinase that activates downstream kinases JNK and p38 in response to cellular stress, cytokines, or growth factors, regulating processes like apoptosis, differentiation, and inflammation. Researchers use MAP2K4 antibodies to detect and quantify endogenous MAP2K4 protein levels in techniques such as Western blotting, immunohistochemistry, and immunofluorescence. These antibodies typically target specific epitopes, often within the kinase domain or regulatory regions, and help investigate post-translational modifications (e.g., phosphorylation at Ser257/Thr261) critical for its activation.
MAP2K4 has been implicated in cancer, neurodegenerative diseases, and developmental disorders, with its dysregulation linked to tumor suppression loss or metastatic progression. Antibodies against MAP2K4 enable studies exploring its role in signaling crosstalk, drug resistance, and therapeutic targeting. Validation of antibody specificity is essential, as splice variants or homologous kinases may cause cross-reactivity. Commercial MAP2K4 antibodies are available in monoclonal and polyclonal forms, derived from hosts like rabbit or mouse, with applications spanning basic research and clinical biomarker analysis. Understanding MAP2K4's mechanistic contributions via these antibodies provides insights into disease pathways and potential intervention strategies.
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