| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FBXO44 |
| Uniprot No | Q9H4M3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-255aa |
| 氨基酸序列 | MAVGNINELPENILLELFTHVPARQLLLNCRLVCSLWRDLIDLVTLWKRKCLREGFITEDWDQPVADWKIFYFLRSLHRNLLHNPCAEEGFEFWSLDVNGGDEWKVEDLSRDQRKEFPNDQVKKYFVTSYYTCLKSQVVDLKAEGYWEELMDTTRPDIEVKDWFAARPDCGSKYQLCVQLLSSAHAPLGTFQPDPATIQQKSDAKWREVSHTFSNYPPGVRYIWFQHGGVDTHYWAGWYGPRVTNSSITIGPPLP |
| 预测分子量 | 37.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是模拟生成的关于FBXO44重组蛋白的参考文献(实际文献需通过学术数据库验证)：
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1. **文献名称**：*"Structural and functional analysis of FBXO44. a component of the SKP1-CUL1-F-box protein complex"*
**作者**：Zhang Y, et al.
**摘要**：本研究利用重组表达的FBXO44蛋白解析其晶体结构，揭示其F-box结构域与SKP1的结合模式，并证明其在泛素化通路中通过识别特定底物(如p21)调控细胞周期进程。
2. **文献名称**：*"FBXO44 mediates ubiquitination and degradation of p53 in cancer cells"*
**作者**：Wang L, et al.
**摘要**：通过体外重组实验发现，FBXO44作为E3泛素连接酶复合物的适配蛋白，可直接结合并泛素化肿瘤抑制因子p53.促进其蛋白酶体降解，提示其在癌症中的潜在作用。
3. **文献名称**：*"Recombinant FBXO44 characterization and its role in DNA damage response"*
**作者**：Kimura T, et al.
**摘要**：利用重组FBXO44蛋白进行功能筛选，发现其通过调控ATR-CHK1信号通路参与DNA损伤修复，为FBXO44在基因组稳定性中的作用提供证据。
4. **文献名称**：*"FBXO44 interacts with HDAC6 to modulate cellular autophagy"*
**作者**：Garcia-Ruiz S, et al.
**摘要**：重组蛋白互作实验证实FBXO44与组蛋白去乙酰化酶HDAC6结合，通过泛素化修饰调控自噬相关蛋白LC3的稳定性，影响细胞自噬过程。
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**注意**：以上文献为模拟生成，实际研究需查阅PubMed、Web of Science等平台(关键词：FBXO44. recombinant protein, ubiquitination)。FBXO44相关研究较少，可拓展至F-box蛋白家族或SCF复合物的文献。
FBXO44 is a member of the F-box protein family, which serves as a substrate-recognition component of the Skp1-Cullin-F-box (SCF) ubiquitin ligase complex. This complex plays a central role in the ubiquitin-proteasome system (UPS), targeting specific proteins for degradation by tagging them with ubiquitin chains. FBXO44 contains a conserved F-box domain that mediates interactions with Skp1. linking it to the core SCF machinery. Unlike some F-box proteins, FBXO44 lacks canonical substrate-binding motifs like leucine-rich repeats (LRRs) or WD40 domains, suggesting a unique mechanism for substrate recognition, possibly through auxiliary adaptors or post-translational modifications.
Recombinant FBXO44 protein is typically produced in heterologous expression systems (e.g., *E. coli*, insect cells) to study its biochemical properties, structural features, and interactions. Its recombinant form enables researchers to explore its role in cellular processes such as cell cycle regulation, apoptosis, and stress responses. Studies suggest FBXO44 may regulate transcription factors or signaling molecules, though its precise physiological substrates remain poorly characterized. Dysregulation of FBXO44 has been implicated in cancer progression, with evidence linking its overexpression to tumor growth and metastasis in certain contexts.
Structural studies using recombinant FBXO44 aim to resolve its 3D architecture and binding interfaces, which could inform drug discovery efforts targeting SCF complexes. Challenges in working with recombinant FBXO44 include maintaining solubility and stability due to its intrinsically disordered regions. Current research focuses on identifying its interaction partners, regulatory mechanisms, and potential therapeutic relevance in diseases tied to UPS dysfunction.
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