| 纯度 | > 95 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | AIF1L |
| Uniprot No | Q9BQI0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-150aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMSGELSNRFQGGKAFGLLKARQERRLAEIN REFLCDQKYSDEENLPEKLTAFKEKYMEFDLNNEGEIDLMSLKRMMEKLG VPKTHLEMKKMISEVTGGVSDTISYRDFVNMMLGKRSAVLKLVMMFEGKA NESSPKPVGPPPERDIASLP |
| 预测分子量 | 19 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AIF1L重组蛋白的参考文献示例(内容为模拟创作，仅供参考)：
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1. **标题**: *"Cloning and Prokaryotic Expression of Recombinant AIF1L: Implications for Inflammatory Signaling"*
**作者**: Zhang Y, et al.
**摘要**: 本研究成功克隆了AIF1L基因，并在大肠杆菌中实现了重组蛋白的可溶性表达。纯化后的蛋白通过体外实验证实可激活巨噬细胞的NF-κB通路，提示其在炎症反应中的潜在作用。
2. **标题**: *"Structural Characterization of AIF1L Reveals a Calcium-Binding Domain Critical for Immune Function"*
**作者**: Kumar S, et al.
**摘要**: 通过X射线晶体学解析了AIF1L重组蛋白的三维结构，发现其含有保守的EF-hand钙离子结合域。功能实验表明，钙离子结合能力是AIF1L调控T细胞活化的关键因素。
3. **标题**: *"AIF1L Recombinant Protein Attenuates Neuroinflammation in a Mouse Model of Alzheimer’s Disease"*
**作者**: Li H, et al.
**摘要**: 利用哺乳动物细胞系表达的重组AIF1L蛋白，在小鼠模型中验证了其通过抑制小胶质细胞过度活化减轻神经炎症的作用，为神经退行性疾病治疗提供新靶点。
4. **标题**: *"High-Yield Purification and Functional Analysis of AIF1L in Vascular Smooth Muscle Cells"*
**作者**: Wang X, et al.
**摘要**: 开发了一种高效层析法纯化AIF1L重组蛋白，并证明其在血管平滑肌细胞迁移中的促动脉粥样硬化功能，可能与ROS信号通路相关。
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**注**：以上文献为示例，实际研究中请通过PubMed、Google Scholar等平台检索真实文献(关键词如“AIF1L recombinant protein”或“AIF1L expression”)。若需具体文章，建议补充更详细的研究方向或应用场景。
AIF1L (Allograft Inflammatory Factor 1-Like) is a calcium-binding protein encoded by the *AIF1L* gene, sharing structural homology with AIF1 (Allograft Inflammatory Factor 1), a key regulator of inflammatory responses. First identified through genomic analyses, AIF1L is characterized by two conserved EF-hand domains that enable calcium ion binding, a feature linked to its functional versatility in cellular processes. While AIF1 is predominantly associated with immune activation and macrophage function, AIF1L exhibits broader tissue expression, including the brain, testes, and immune cells, suggesting distinct biological roles.
Recombinant AIF1L protein is engineered using heterologous expression systems, such as *E. coli* or mammalian cell lines, to produce purified, functional protein for research. Its recombinant form retains calcium-binding capacity and structural stability, enabling studies on its interaction partners and signaling pathways. Emerging evidence implicates AIF1L in cytoskeletal organization, apoptosis modulation, and immune regulation. For instance, it may influence T-cell activation and neuroinflammatory responses by interacting with proteins involved in membrane dynamics or calcium signaling.
Research on AIF1L remains exploratory, with potential implications in neurodegenerative diseases, autoimmune disorders, and cancer. Its recombinant form serves as a critical tool for *in vitro* assays, antibody production, and structural studies to decipher its mechanistic role. Despite functional overlaps with AIF1. AIF1L’s unique expression patterns and putative roles in non-immune tissues highlight its importance as a standalone research target. Further characterization of AIF1L could unveil novel pathways in cellular homeostasis and disease, positioning it as a potential biomarker or therapeutic candidate.
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