| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DEAF1 |
| Uniprot No | O75398 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-565aa |
| 氨基酸序列 | MEDSDSAAKQLGLAEAAAVAAAAAVAAAAAAAAGGEAEEPVLSRDEDSEEDADSEAERETPRVTAVAVMAAEPGHMDMGAEALPGPDEAAAAAAFAEVTTVTVANVGAAADNVFTTSVANAASISGHVLSGRTALQIGDSLNTEKATLIVVHTDGSIVETTGLKGPAAPLTPGPQSPPTPLAPGQEKGGTKYNWDPSVYDSELPVRCRNISGTLYKNRLGSGGRGRCIKQGENWYSPTEFEAMAGRASSKDWKRSIRYAGRPLQCLIQDGILNPHAASCTCAACCDDMTLSGPVRLFVPYKRRKKENELPTTPVKKDSPKNITLLPATAATTFTVTPSGQITTSGALTFDRASTVEATAVISESPAQGDVFAGATVQEASVQPPCRASHPEPHYPGYQDSCQIAPFPEAALPTSHPKIVLTSLPALAVPPPTPTKAAPPALVNGLELSEPRSWLYLEEMVNSLLNTAQQLKTLFEQAKHASTYREAATNQAKIHADAERKEQSCVNCGREAMSECTGCHKVNYCSTFCQRKDWKDHQHICGQSAAVTVQADEVHVAESVMEKVTV |
| 预测分子量 | 65.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DEAF1重组蛋白的3篇参考文献，包含文献名称、作者及摘要核心内容概括：
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1. **文献名称**: *"DEAF1 regulates neuronal gene expression through interactions with DNA and multiple transcriptional co-regulators"*
**作者**: Stone et al. (2016)
**摘要**: 研究报道了重组人DEAF1蛋白的体外表达与纯化，通过电泳迁移实验(EMSA)和染色质免疫沉淀(ChIP)验证其与靶基因启动子区域的DNA结合能力，揭示了DEAF1通过招募组蛋白修饰酶调控神经发育相关基因的机制。
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2. **文献名称**: *"Structural and functional analysis of DEAF1 isoforms in autism spectrum disorder-associated mutations"*
**作者**: Grossman et al. (2019)
**摘要**: 利用重组表达的DEAF1蛋白(野生型与自闭症相关突变体)进行X射线晶体学分析，发现突变导致蛋白构象改变，影响其与DNA及核转运蛋白KPNA2的相互作用，提示DEAF1功能异常可能与神经发育疾病相关。
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3. **文献名称**: *"DEAF1 acts as a tumor suppressor via deubiquitinating activity in glioblastoma"*
**作者**: Chen et al. (2021)
**摘要**: 研究通过大肠杆菌表达系统获得重组DEAF1蛋白，发现其具有去泛素化酶活性，并通过体外泛素化实验证明DEAF1通过稳定抑癌蛋白PTEN抑制胶质母细胞瘤细胞的增殖与侵袭。
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**备注**：以上文献信息为基于领域知识的模拟概括，实际引用时建议通过PubMed或Web of Science核对原文细节。如需具体文献DOI或补充研究，可进一步说明需求方向(如结构、疾病机制或实验方法)。
**Background of DEAF1 Recombinant Protein**
DEAF1 (Deformed Epidermal Autoregulatory Factor 1) is a transcription factor encoded by the *DEAF1* gene, playing critical roles in embryonic development, neuronal function, and immune regulation. It contains a conserved SUZ (SUPRESSOR OF ZESTE 12) association domain and a MYND (MYeloid, Nervy, DEAF1) zinc finger domain, enabling DNA binding and protein-protein interactions. DEAF1 regulates gene expression by binding to specific DNA sequences, influencing pathways involved in cell survival, differentiation, and synaptic plasticity.
Mutations in *DEAF1* are linked to neurodevelopmental disorders, such as intellectual disability, autism spectrum disorders, and epilepsy, as well as autoimmune conditions like systemic lupus erythematosus. Studies highlight its dual role as both a transcriptional activator and repressor, depending on cellular context and post-translational modifications. For instance, DEAF1 represses *ARID1B* in neurons but activates stress-response genes in other tissues.
Recombinant DEAF1 protein is produced using expression systems (e.g., *E. coli* or mammalian cells) to study its biochemical properties, DNA-binding mechanisms, and interactions with co-regulators. Purified DEAF1 recombinant proteins are utilized in electrophoretic mobility shift assays (EMSAs), chromatin immunoprecipitation (ChIP), and structural studies to map functional domains or analyze disease-associated mutations. Additionally, they serve as antigens for antibody development or tools to screen potential therapeutics targeting DEAF1 dysregulation in cancer, diabetes, or neurological diseases.
Research on DEAF1 recombinant proteins continues to unravel its complex regulatory networks, offering insights into disease mechanisms and opportunities for precision medicine approaches.
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