| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MGAT4A |
| Uniprot No | Q9UM21 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 93-535aa |
| 氨基酸序列 | LLKELTSKKSLQVPSIYYHLPHLLKNEGSLQPAVQIGNGRTGVSIVMGIPTVKREVKSYLIETLHSLIDNLYPEEKLDCVIVVFIGETDIDYVHGVVANLEKEFSKEISSGLVEVISPPESYYPDLTNLKETFGDSKERVRWRTKQNLDYCFLMMYAQEKGIYYIQLEDDIIVKQNYFNTIKNFALQLSSEEWMILEFSQLGFIGKMFQAPDLTLIVEFIFMFYKEKPIDWLLDHILWVKVCNPEKDAKHCDRQKANLRIRFRPSLFQHVGLHSSLSGKIQKLTDKDYMKPLLLKIHVNPPAEVSTSLKVYQGHTLEKTYMGEDFFWAITPIAGDYILFKFDKPVNVESYLFHSGNQEHPGDILLNTTVEVLPFKSEGLEISKETKDKRLEDGYFRIGKFENGVAEGMVDPSLNPISAFRLSVIQNSAVWAILNEIHIKKATN |
| 预测分子量 | 56 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MGAT4A重组蛋白的模拟参考文献示例(实际文献请通过学术数据库核实)：
1. **标题**：*Recombinant MGAT4A Expression Enhances Branching N-Glycan Synthesis in Cancer Cells*
**作者**：Smith J, et al.
**摘要**：本研究成功克隆并表达了人源MGAT4A重组蛋白，证实其在HEK293细胞中可催化β1.4-N-乙酰葡萄糖胺分支结构的形成，促进肿瘤细胞迁移相关糖基化修饰。
2. **标题**：*Structural and Functional Analysis of MGAT4A Using Recombinant Enzyme*
**作者**：Lee H, et al.
**摘要**：通过大肠杆菌系统表达纯化MGAT4A重组蛋白，解析其酶活动力学参数，并利用突变实验揭示关键催化结构域，为糖基化抑制剂设计提供依据。
3. **标题**：*MGAT4A Knockout and Recombinant Rescue in Metabolic Regulation*
**作者**：Wang Y, et al.
**摘要**：构建MGAT4A敲除小鼠模型，并通过重组蛋白回补实验证明其参与肝细胞胰岛素受体糖基化调控，影响葡萄糖代谢稳态。
4. **标题**：*High-Yield Production of MGAT4A in Insect Cells for Glycoengineering*
**作者**：Zhang R, et al.
**摘要**：采用杆状病毒-昆虫细胞系统高效表达MGAT4A重组蛋白，优化纯化工艺，并应用于体外糖链重塑，提升抗体药物ADCC效应。
**备注**：以上为模拟文献，实际研究需查询PubMed或Google Scholar等平台，建议使用关键词“MGAT4A recombinant”“N-glycosyltransferase expression”进一步检索。
MGAT4A (Mannosyl (Alpha-1.3-)-Glycoprotein Beta-1.4-N-Acetylglucosaminyltransferase, Isozyme A) is a key enzyme in the biosynthesis of N-linked glycans, which play critical roles in protein folding, cell-cell interactions, and immune responses. This Golgi-resident glycosyltransferase catalyzes the transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc to the mannose residue of glycoproteins via a β1.4-linkage, forming branched structures that influence glycoprotein stability and function. MGAT4A-mediated branching is particularly important for regulating biological processes such as cell adhesion, receptor signaling, and pathogen recognition.
The enzyme is expressed in various tissues, including the liver, placenta, and pancreas, with dysregulation linked to diseases like cancer, autoimmune disorders, and congenital disorders of glycosylation. In cancer, elevated MGAT4A activity has been associated with increased metastasis due to enhanced growth factor receptor signaling and immune evasion through altered cell surface glycan patterns.
Recombinant MGAT4A protein is typically produced in mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications and enzymatic activity. Purified recombinant forms enable structural studies, inhibitor screening, and functional assays to dissect its role in glycosylation pathways. Researchers also utilize MGAT4A to engineer glycoforms of therapeutic proteins, as N-glycan branching affects drug efficacy and pharmacokinetics. Current studies focus on developing MGAT4A-specific inhibitors as potential therapeutics and exploring its diagnostic value through glycan biomarker analysis.
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