| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LAMP5 |
| Uniprot No | Q9UJQ1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-280aa |
| 氨基酸序列 | MDLQGRGVPSIDRLRVLLMLFHTMAQIMAEQEVENLSGLSTNPEKDIFVVRENGTTCLMAEFAAKFIVPYDVWASNYVDLITEQADIALTRGAEVKGRCGHSQSELQVFWVDRAYALKMLFVKESHNMSKGPEATWRLSKVQFVYDSSEKTHFKDAVSAGKHTANSHHLSALVTPAGKSYECQAQQTISLASSDPQKTVTMILSAVHIQPFDIISDFVFSEEHKCPVDEREQLEETLPLILGLILGLVIMVTLAIYHVHHKMTANQVQIPRDRSQYKHMG |
| 预测分子量 | 31,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LAMP5重组蛋白的3篇参考文献的简要概括(基于公开研究主题推测，具体文献需通过数据库验证)：
1. **文献名称**：*"LAMP5 regulates dendritic cell function through interaction with MHC class II molecules"*
**作者**：Smith A, et al.
**摘要**：研究揭示了LAMP5重组蛋白在树突状细胞中与MHC II类分子的相互作用，证明其通过调控内体-溶酶体系统影响抗原呈递过程，为免疫治疗提供新靶点。
2. **文献名称**：*"Expression and purification of recombinant human LAMP5 for structural studies"*
**作者**：Chen L, et al.
**摘要**：报道了一种高效的大肠杆菌表达系统，用于制备高纯度LAMP5胞外域重组蛋白，并通过质谱和圆二色光谱验证其正确折叠，为后续功能研究奠定基础。
3. **文献名称**：*"LAMP5 knockdown impairs synaptic vesicle trafficking in hippocampal neurons"*
**作者**：Yamamoto K, et al.
**摘要**：利用重组LAMP5蛋白进行神经元回补实验，发现LAMP5通过调控突触小泡的酸化和递质释放，影响海马神经元突触可塑性，提示其与神经退行性疾病的潜在关联。
**备注**：以上内容为基于LAMP5已知功能(溶酶体调控、免疫与神经作用)的模拟文献摘要，实际文献需通过PubMed/Google Scholar检索关键词“LAMP5 recombinant protein”或“LAMP5 expression”获取。
Lysosome-associated membrane protein 5 (LAMP5) is a member of the LAMP family, which plays critical roles in lysosomal biogenesis, autophagy, and intracellular trafficking. Structurally, LAMP5 shares conserved features with other LAMP proteins, including a heavily glycosylated luminal domain with two N-terminal disulfide-bonded domains and a single transmembrane helix near the C-terminus. Unlike its well-studied relatives LAMP1 and LAMP2. LAMP5 exhibits more restricted tissue expression, with higher abundance in the brain, particularly in specific neuronal populations, and immune cells. This suggests specialized functions in neurobiology and immune regulation.
Recombinant LAMP5 proteins are engineered using expression systems like mammalian cells or *E. coli* to produce purified, biologically active forms for research. These proteins often retain post-translational modifications (e.g., glycosylation) when produced in mammalian systems, which may influence their stability and interactions. Researchers utilize recombinant LAMP5 to study its role in lysosome-phagosome fusion, synaptic vesicle recycling, and potential involvement in neurodegenerative disorders like Alzheimer’s disease, where lysosomal dysfunction is implicated. Its interaction with SNARE proteins and regulators of vesicle trafficking has also drawn attention in membrane dynamics studies.
In cancer research, LAMP5 is investigated as a biomarker due to its aberrant expression in certain tumors. Additionally, recombinant LAMP5 serves as an antigen for antibody development, aiding diagnostic and therapeutic exploration. Despite progress, its precise physiological mechanisms and disease relevance remain under active investigation, highlighting the need for further functional studies using recombinant tools.
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