| 纯度 | >90%SDS-PAGE. |
| 种属 | Mycobacterium |
| 靶点 | hbhA |
| Uniprot No | P9WIP8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-199aa |
| 氨基酸序列 | AENSNIDDIKAPLLAALGAADLALATVNELITNLRERAEETRTDTRSRVEESRARLTKLQEDLPEQLTELREKFTAEELRKAAEGYLEAATSRYNELVERGEAALERLRSQQSFEEVSARAEGYVDQAVELTQEALGTVASQTRAVGERAAKLVGIELPKKAAPAKKAAPAKKAAPAKKAAAKKAPAKKAAAKKVTQK |
| 预测分子量 | 28.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于hbhA重组蛋白的3篇代表性文献示例(注：文献信息为示例性概括，建议通过学术数据库核实具体内容)：
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1. **文献名称**: *Heparin-binding hemagglutinin (HBHA) of Mycobacterium tuberculosis: a key adhesin in bacterial pathogenesis*
**作者**: Menozzi FD, et al.
**摘要**: 研究揭示了hbhA编码的肝素结合血凝素(HBHA)在结核分枝杆菌粘附宿主细胞中的作用，通过重组蛋白实验证明HBHA通过结合宿主表面硫酸乙酰肝素介导细菌粘附，并可能影响感染的早期阶段。
2. **文献名称**: *Recombinant HBHA protein enhances Th1 immune response in a murine tuberculosis model*
**作者**: Hamasur B, et al.
**摘要**: 利用重组hbhA蛋白评估其免疫原性，发现该蛋白可诱导小鼠产生强烈的Th1型免疫反应，提示其作为结核病亚单位疫苗候选分子的潜力。
3. **文献名称**: *Structural and functional analysis of the Mycobacterium tuberculosis HBHA protein*
**作者**: Pethe K, et al.
**摘要**: 通过重组表达hbhA蛋白并结合晶体结构分析，揭示了HBHA的甲基化修饰对其与宿主细胞表面受体的结合能力至关重要，为靶向该蛋白的抗结核策略提供结构基础。
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**建议**：如需真实文献，可在PubMed或Google Scholar检索以下关键词：
`hbhA recombinant protein Mycobacterium tuberculosis`、`HBHA adhesion vaccine`
示例文献年份多集中于2000-2010年，近期研究可能涉及hbhA在诊断或免疫调控中的新应用。
**Background of HBHA Recombinant Protein**
HBHA (Heparin-Binding Hemagglutinin) is a surface-exposed adhesin primarily associated with *Mycobacterium tuberculosis*, the causative agent of tuberculosis (TB). This 28-kDa protein plays a critical role in bacterial pathogenesis by mediating adherence to host epithelial cells and facilitating extrapulmonary dissemination. HBHA binds heparin sulfate proteoglycans on host cell surfaces, promoting bacterial colonization and immune evasion. Its structural features include a C-terminal lysine-rich domain responsible for heparin binding and a coiled-coil N-terminal region involved in oligomerization.
Recombinant HBHA (rHBHA) is produced via genetic engineering, often using *E. coli* or yeast expression systems. Purification typically involves affinity chromatography, leveraging histidine tags or heparin-binding properties. rHBHA retains the immunogenic and functional characteristics of native HBHA, making it valuable for research and applications.
Studies highlight rHBHA’s role in TB diagnostics and vaccine development. It serves as an antigen in serological assays to distinguish latent TB from active disease, improving diagnostic specificity. Additionally, rHBHA-based subunit vaccines show promise in preclinical models by eliciting protective immune responses against *M. tuberculosis*. Its immunomodulatory properties also aid in studying host-pathogen interactions, particularly mechanisms of immune evasion.
Recent research explores rHBHA’s potential as a biomarker for TB progression and treatment monitoring. Challenges include optimizing stability and scalability for clinical use. Overall, rHBHA remains a pivotal tool in advancing TB research, bridging gaps in understanding pathogenesis and therapeutic innovation.
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